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描述 概述 Recombinant, rat FPTase composed of an α- and a β-subunit (74 and 46 kDa, respectively). The α-subunit is expressed as a GST-fusion construct with an N-terminal His•Tag® sequence. Both the GST moiety and the His•Tag® sequence can be utilized to facilitate enzyme capturing in prenylation and binding studies or, when desired, can also be cleaved off by TEV protease. Requires Zn2+ and Mg2+ for optimal activity. 产品目录编号 344146 品牌系列 Calbiochem®
同义词 FTase, Farnesyl Proteintransferase 参考 参考 Zimmerman, K.K., et al. 1998. Protein Expr. Purif. 14, 395.
Reiss, Y., et al. 1990. Cell 62, 81.产品信息 形式 Liquid 配方 In 40 mM NaCl, 25 mM HEPES, 5 mM DTT, pH 7.2. Molar Mass 120000 生物信息 生物活性 1 pmol FTase will transfer ≥1 pmol farnesyl to H-Ras in 10 min at 37°C, pH 7.2. 纯度 ≥90% by SDS-PAGE 储存和货运信息 货运代码 Dry Ice Only
毒性 Standard Handling
储存 ≤ -70°C
避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
特殊指南 Following initial thaw, aliquot and freeze (-70°C).
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