merck millipore,默克密理博,17-10262,ChIPAb+™ JMJD1C - ChIP Validated Antibody and Primer Set

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merck millipore,默克密理博,17-10262,ChIPAb+™ JMJD1C - ChIP Validated Antibody and Primer Set
产品名称：ChIPAb+™ JMJD1C - ChIP Validated Antibody and Primer Set
产品型号：17-10262
This ChIPAb+ JMJD1C -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.

merck millipore,默克密理博,17-10262,ChIPAb+™ JMJD1C - ChIP Validated Antibody and Primer Set

产品介绍

merck millipore,默克密理博,17-10262,ChIPAb+™ JMJD1C - ChIP Validated Antibody and Primer Set

重要规格表

品种反应性	主要应用
Rhesus Macaque, H, Rb, Chp	ChIP, WB, ICC

描述
产品目录编号	17-10262
商名	Upstate ChIPAb+
描述	ChIPAb+™ JMJD1C - ChIP Validated Antibody and Primer Set
概述	All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction. The ChIPAb+JMJD1C set includes the JMJD1C antibody, a Normal Rabbit IgG, and control primers which amplify a 110 bp region of ChIP Primers, human β-globin. The JMJD1C and negative controls are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of JMJD1C -associated chromatin.
Alternate Names	TR-interacting protein 8 JMJD jumonji domain containing 1C thyroid receptor interacting protein 8 probable JmjC domain-containing histone demethylation protein 2C thyroid hormone receptor interactor 8 Thyroid receptor-interacting protein 8 Jumonji domain-containing protein 1C
背景信息	JMJD1C (jumonji domain-containing protein 1C) is also known as TRIP8 and mutations in this histone demethylase have been linked to certain forms of autism. JMJD1C is also thought to work in reactivating genes in embryonic stem cells, pancreatic islet and gastric cancer. JMJD1C has also been implicated as a gene expression regulator. JMJD1C with WHISTLE have recently been shown to also play a role in mouse testis development. JMJD1C has been identified as a nuclear-receptor coactivator and is a candidate gene for autism.

产品信息
格式	Affinity Purified
控制	Includes normal rabbit IgG and primers specific for human β-globin.
演示	Anti-JMJD1C (rabbit polyclonal). One vial containing 50 µL of purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl and 0.05% sodium azide before the addition of glycerol to 30%. Store at -20°C. Concentration: 0.7 mg/mL Normal Rabbit IgG. One vial containing 125 µg of Rabbit IgG in 125 µL of storage buffer containing 0.05% sodium azide. Store at -20°C. ChIP Primers, human β-globin. One vial containing 75 μL of each primer (5 μM) specific for the human β-globin promoter. Store at -20°C. FOR: AGG ACA GGT ACG GCT GTC ATC REV: TTT ATG CCC AGC CCT GGC TC

应用
应用	This ChIPAb+ JMJD1C -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
主要应用	Chromatin Immunoprecipitation (ChIP) Western Blotting Immunocytochemistry
应用说明	Chromatin Immunoprecipitation: Representative lot data. Sonicated chromatin prepared from HeLa cells (1 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 1.4 µg of either Normal Rabbit IgG or Anti-JMJD1C and the Magna ChIP™ A Kit (Cat. # 17-610). Successful immunoprecipitation of JMJD1C associated DNA fragments was verified by qPCR using ChIP Primers, human β-globin as a positive locus, and β-actin promoter primers as a negative locus. (Figure 2). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated. Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details. Western Blot Analysis: Representative lot data. HeLa nuclear extract was probed with Anti-JMJD1C (1 μg/mL). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates JMJD1C (~284 kDa). (Figure 3). Immunocytochemistry Analysis: Representative lot data. Confocal fluorescent analysis of HeLa cells using Anti-JMJD1C (Red). Actin filaments have been labeled with Alexa Fluor® 488 dye -Phalloidin (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus. (Figure 4).

生物信息
免疫原品种	KLH-conjugated linear peptide corresponding to human JMJD1C.
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
品种反应性	Rhesus MacaqueHumanRabbitChimpanzee
Species Reactivity Note	Demonstrated to react with human. Predicted to react with rabbit, chimpanzee, rhesus macaque, and orangutan based on 100% sequence homology.
抗体类型	Polyclonal Antibody
Entrez基因编号	NP_116165
基因符号	JHDM2C TRIP8 RP11-10C13.2 JMJD1C
纯化方法	Affinity purified
UniProt编号	Q15652
UniProt汇总	FUNCTION: Probable histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Demethylation of Lys residue generates formaldehyde and succinate. May be involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes (By similarity). COFACTOR: Binds 1 Fe2+ ion per subunit (By similarity). SUBUNIT STRUCTURE: Interacts specifically with the ligand-binding domain of the thyroid receptor (TR). Requires the presence of thyroid hormone for its interaction. SUBCELLULAR LOCATION: Nucleus (By similarity). DOMAIN: Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs are known to mediate the association with nuclear receptors (By similarity). PTM: Phosphorylated upon DNA damage, probably by ATM or ATR. SEQUENCE SIMILARITIES: Belongs to the JHDM2 histone demethylase family. Contains 1 JmjC domain. SEQUENCE CAUTION: The sequence AAC41741.1 differs from that shown. Reason: Frameshift at position 2171. The sequence CAD97921.1 differs from that shown. Reason: Frameshift at position 1697. The sequence CAI10947.1 differs from that shown. Reason: Erroneous gene model prediction.

产品使用声明
质量保证	Chromatin Immunoprecipitation: Representative lot data. Sonicated chromatin prepared from HeLa cells (1 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 1.4 µg of either Normal Rabbit IgG, or Anti-JMJD1C and the Magna ChIP™ A Kit (Cat. # 17-610). Successful immunoprecipitation of JMJD1C associated DNA fragments was verified by qPCR using ChIP Primers, human β-globin (Figure 1). Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品使用声明

质量保证

Chromatin Immunoprecipitation:
Representative lot data.
Sonicated chromatin prepared from HeLa cells (1 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 1.4 µg of either Normal Rabbit IgG, or Anti-JMJD1C and the Magna ChIP™ A Kit (Cat. # 17-610).
Successful immunoprecipitation of JMJD1C associated DNA fragments was verified by qPCR using ChIP Primers, human β-globin (Figure 1).
Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

包装信息
数量	25 assays
包装	25 assays per set. Recommended use: 1.4 µg (2 µL) of antibody per chromatin immuno-precipitation (dependent upon biological context).