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纯度 ≥97% by SDS-PAGE 描述 概述 Recombinant, human basic fibroblast growth factor expressed in E. coli. Potent mitogen for bone cells. Inhibits DNA synthesis and alkaline phosphatase activity in rat osteosarcoma cell lines. Has been implicated in the development of Kaposi sarcoma in vitro. Inhibits inducible NOS in bovine retinal pigmented epithelial cells. 产品目录编号 341595 品牌系列 Calbiochem®
同义词 rhbFGF, Fibroblast Growth Factor, Basic, Human, Recombinant, E. coli 参考 参考 Goureau, O., et al. 1995. Eur. J. Biochem. 230, 1046.
Gibran, N.S., et al. 1994. J. Surg. Res. 56, 226.
Li, J.J., et al. 1993. Cancer 72, 2253.
Mignatti, P., et al. 1992. J. Cell Physiol. 151, 81.
Mignatti, P., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 11007.
Baird, A., and Klagsbrun, M. 1991. Cancer Cells 3, 239.
Gospodarowicz, D. 1991. Ann. NY Acad. Sci. 638, 1.
Basilico, C., et al. 1989. Ann. NY Acad. Sci. 567, 95.
Burgess, W.H., and Maciag, T. 1989. Annu. Rev. Biochem. 58, 575.
Rogelj, S., et al. 1989. J. Cell. Biochem. 39, 13.
Rizzino, A., et al. 1988. Cancer Res. 48, 4266.
Abraham, J.A., et al. 1986. EMBO J. 5, 2523.产品信息 CAS号 106096-93-9 形式 Lyophilized 配方 Lyophilized from sterile-filtered 20 mM Tris-HCl, 1 M NaCl, 50 µg BSA/1 µg cytokine, pH 7.0. Molar Mass 16400 生物信息 生物活性 ED₅₀ = 100-250 pg/ml as monitored in a mitogenic assay by measuring FGF basic-dependent ³H-thymidine incorporation in quiescent NR6R/3T3 fibroblasts 纯度 ≥97% by SDS-PAGE 理化信息 污染物 Endotoxin: ≤1 EU/µg FGF 溶解度 Sterile PBS containing 1 mM DTT and ≥0.1% HSA or BSA GHS安全信息 RTECS LK3641325 储存和货运信息 货运代码 Ambient Temperature Only
毒性 Standard Handling
储存 ≤ -70°C
无需冷冻 No
特殊指南 Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 6 months at -70°C.
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