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Key Applications PCU 描述 产品目录编号 2114 品牌系列 Chemicon®
商名 - Chemicon
描述 Detergent-OUT™ Micro Kit 概述 The Chemicon Detergent-OUT Kit is a simple high performance method for removing detergents from protein solutions. Removes detergents without significant dilution of protein solution. Simply load the protein solution on the Detergent-OUT Spin column and collect detergent free protein solution. Micro columns are suitable for removing detergent from a maximum of 0.2 mL sample volume. 产品信息 部件 - Collection Tubes (2 mL) 10
- Detergent-OUT Columns-DTG-100X 10 Micro
应用 应用 The Detergent-OUT Kit is a simple high performance method for removing detergents from protein solutions. 主要应用 - Protein Clean-up
应用说明 PROTOCOL:
1. Invert the column several times to re-suspend the resin material. Remove the tip of the column and let the liquid drain off.
2. Buffer Equilibration: SpinOUT columns are supplied in deionized water. Equilibrate the column with a buffer of your choice, as follows. Note: Do not equilibrate the columns with Tris buffer. Apply about 0.5 mL of a desired buffer into the column. Let the buffer drain into collection tube. Repeat this process 3 times.
3. Place the column in a 2.0 mL micro-centrifuge tube. Centrifuge at 1000 xg for 20-30 seconds. Discard the liquid collected in the centrifuge tube.
4. Place the column back in the same centrifuge tube. Carefully apply the protein suspension to the center of the column. Wait for 1-2 minutes. Note: If your protein solution is in Tris buffer, exchange the protein solution buffer to phosphate or other buffer, using either a SpinOUT column or by dialysis.
5. After loading the column centrifuge at 1000 xg for 20-30 seconds. Collect the liquid comprising purified protein material free from detergent originally present in the sample.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Store at 2-8°C for up to 6 months from date of purchase. To avoid possible microbial contamination, dispense all solutions aseptically. 包装信息 数量 1 kit 包装 10 columns
上一件merck millipore产品：merck millipore,默克密理博,14-1059,Human Recombinant Hp1α (CBX5)
下一件merck millipore产品：merck millipore,默克密理博,390238,Camptothecin, 10-Hydroxy-, Camptotheca acuminata - CAS 64439-81-2 - Calbiochem
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