merck millipore,默克密理博,04-469,Anti-SREBP1 Antibody, clone 2121

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merck millipore,默克密理博,04-469,Anti-SREBP1 Antibody, clone 2121
产品名称：Anti-SREBP1 Antibody, clone 2121
产品型号：04-469
Anti-SREBP1 Antibody, clone 2121 is a Mouse Monoclonal Antibody for detection of SREBP1 also known as sterol regulatory element binding transcription factor 1 & has been validated in WB.

merck millipore,默克密理博,04-469,Anti-SREBP1 Antibody, clone 2121

产品介绍

merck millipore,默克密理博,04-469,Anti-SREBP1 Antibody, clone 2121

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
H	WB	M	Purified	Monoclonal Antibody

描述
产品目录编号	04-469
品牌系列	Upstate
商名	Upstate
描述	Anti-SREBP1 Antibody, clone 2121
Alternate Names	SREBF1, sterol regulatory element binding transcription factor 1 SREBF1

产品信息
格式	Purified
演示	100 μL (1 mg/mL) of protein A purified mouse monoclonal IgG1 in PBS with 0.1% sodium azide.

应用
应用	Anti-SREBP1 Antibody, clone 2121 is a Mouse Monoclonal Antibody for detection of SREBP1 also known as sterol regulatory element binding transcription factor 1 & has been validated in WB.
主要应用	Western Blotting

生物信息
免疫原品种	Recombinant His-tagged human SREBP-1a protein encompassing amino acids 301-407.
克隆	2121
宿主	Mouse
特异性	Reacts with the basic helix-loop-helix leucine zipper (bHLH-ZIP) of SREBP.
同种型	IgG1
品种反应性	Human
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_004176.3 NM_001005291.1
Entrez基因汇总	This gene encodes a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. The protein is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Two transcript variants encoding different isoforms have been found for this gene.
基因符号	SREBP-1 SREBF1 SREBP1
纯化方法	Protein A purfied
UniProt编号	P36956
UniProt汇总	FUNCTION: SwissProt: P36956 # Transcriptional activator that binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity, binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3'). Regulates the transcription of genes for sterol biosynthesis and the LDL receptor gene. SIZE: 1147 amino acids; 121645 Da SUBUNIT: Forms a tight complex with SCAP in the ER membrane. Efficient DNA binding of the soluble transcription factor fragment requires dimerization with another bHLH protein. SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Note=Moves from the endoplasmic reticulum to the Golgi in the absence of sterols. TISSUE SPECIFICITY: Expressed in a wide variety of tissues, most abundant in liver and adrenal gland. In fetal tissues lung and liver shows highest expression. Isoform SREBP-1C predominates in liver, adrenal gland and ovary, whereas isoform SREBP-1A predominates in hepatoma cell lines. Isoform SREBP-1A and isoform SREBP-1C are found in kidney, brain, white fat, and muscle. PTM: Under sterol-depleted conditions, SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage occurs within the luminal loop and is directly regulated by sterols. The second cleavage by site-2 protease occurs within the first transmembrane stretch and liberates the soluble transcription factor. Cleavage by the cysteine proteases, caspase- 3 and caspase-7, is induced during apoptosis, independent of sterol levels. SIMILARITY: SwissProt: P36956 ## Contains 1 basic helix-loop-helix (bHLH) domain. MISCELLANEOUS: SREBPs have to be in a complex with the cleavage- activating protein (SCAP) to move to the Golgi and be cleaved by site-1 protease.

产品使用声明
质量保证	Routinely evaluated by immunoblot.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.