merck millipore,默克密理博,04-1117,Anti-alpha Tubulin Antibody, clone EP1332Y, rabbit monoclonal

搜索"Merck Millipore 密理愽"产品

Merck Millipore 默克密理愽

当前位置：首页 > >Merck Millipore > 生命科学研究
生命科学研究

merck millipore,默克密理博,04-1117,Anti-alpha Tubulin Antibody, clone EP1332Y, rabbit monoclonal
产品名称：Anti-alpha Tubulin Antibody, clone EP1332Y, rabbit monoclonal
产品型号：04-1117
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-alpha Tubulin Antibody, clone EP1332Y, Rab More>>

merck millipore,默克密理博,04-1117,Anti-alpha Tubulin Antibody, clone EP1332Y, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-1117,Anti-alpha Tubulin Antibody, clone EP1332Y, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	ICC, WB	Rb	Unpurified	Monoclonal Antibody

描述
产品目录编号	04-1117
描述	Anti-alpha Tubulin Antibody, clone EP1332Y, rabbit monoclonal
Alternate Names	tubulin, alpha, brain-specific Alpha-tubulin 3 Tubulin B-alpha-1 Tubulin alpha-3 chain tubulin, alpha 1a tubulin, alpha 3
背景信息	Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.

产品信息
格式	Unpurified
控制	HeLa cell lysate.
演示	Rabbit Monoclonal in buffer containing 50 mM Tris-glycine (pH 7.4), 0.18 M NaCl containing 40% glycerol, 0.25% sodium azide, 0.25 mM EDTA, 25 mM citric acid, and 0.5% BSA.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-alpha Tubulin Antibody, clone EP1332Y, Rabbit is validated for use in IH(P), WB, IC, IP for the detection of alpha Tubulin.
主要应用	Immunocytochemistry Western Blotting
应用说明	Immunohistochemistry Analysis: 1:250 - 500 dilution from a previous lot detected alpha Tubulin in human gastric carcinoma tissue. Immunocytochemistry Analysis: 1:250 - 500 dilution from a previous lot detected alpha Tubulin in HeLa cell lysate. Immunoprecipitation: 1:50 dilution from a previous lot was used on IP. Flow Cytometry: 1:20 dilution from a previous lot was used on FC.

生物信息
免疫原品种	Synthetic peptide corresponding to residues near the N-terminus of human alpha Tubulin.
表位	N-terminus
克隆	EP1332Y
宿主	Rabbit
特异性	This antibody recognizes alpha Tubulin.
同种型	IgG
品种反应性	RatHumanMouse
Species Reactivity Note	Human, mouse, and rat.
抗体类型	Monoclonal Antibody
Entrez基因编号	P68363
基因符号	hum-a-tub2 B-ALPHA-1 FLJ25113 LIS3 TUBA3 hum-a-tub1
纯化方法	Unpurified
UniProt编号	Q71U36
UniProt汇总	FUNCTION: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. SUBUNIT STRUCTURE: Dimer of alpha and beta chains. TISSUE SPECIFICITY: Expressed at a high level in fetal brain. PTM: Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively By similarity. INVOLVEMENT IN DISEASE: Defects in TUBA1A are the cause of lissencephaly type 3 (LIS3) [MIM:611603]. LIS is characterized by a smooth brain surface due to the absence (agyria) or reduction (pachygyria) of surface convolutions. It is often associated with psychomotor retardation and seizures. LIS3 features include agyria or pachygyria or laminar heterotopia, severe mental retardation, motor delay, variable presence of seizures, and abnormalities of corpus callosum, hippocampus, cerebellar vermis and brainstem. SEQUENCE SIMILARITIES: Belongs to the tubulin family.

产品使用声明
质量保证	Evaluated by Western Blot on HeLa cell lysate. Western Blot Analysis: 1:50,000 dilution of antibody was used to detect alpha Tubulin in HeLa cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.