merck millipore,默克密理博,17-646,ChIPAb+ HP1γ - ChIP Validated Antibody and Primer Set

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merck millipore,默克密理博,17-646,ChIPAb+ HP1γ - ChIP Validated Antibody and Primer Set
产品名称：ChIPAb+ HP1γ - ChIP Validated Antibody and Primer Set
产品型号：17-646
This ChIPAb+ HP1γ -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.

merck millipore,默克密理博,17-646,ChIPAb+ HP1γ - ChIP Validated Antibody and Primer Set

产品介绍

merck millipore,默克密理博,17-646,ChIPAb+ HP1γ - ChIP Validated Antibody and Primer Set

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
Vrt, H, M	ChIP, WB	M	Ascites	Monoclonal Antibody

描述
产品目录编号	17-646
品牌系列	Upstate
商名	Upstate ChIPAb+
描述	ChIPAb+ HP1γ - ChIP Validated Antibody and Primer Set
概述	All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction. The ChIPAb+ HP1γ antibody/primer set includes the HP1g antibody, a negative control antibody (purified mouse IgG), and qPCR primers which amplifies a 213 bp region within the coding region of the human GAPDH gene. The HP1γ and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of HP1γ associated chromatin.
Alternate Names	heterochromatin-like protein 1 HP1 gamma HP1 gamma homolog Heterochromatin protein 1 homolog gamma Modifier 2 protein chromobox homolog 3 chromobox homolog 3 (Drosophila HP1 gamma) chromobox homolog 3 (HP1 gamma homolog, Drosophila) heterochromatin protein HP1 gamma

产品信息
格式	Ascites
控制	Includes negative control mouse IgG antibody and primers specific for of human GAPDH coding region.
演示	HP1γ (mouse monoclonal IgG). One vial containing 125 μL of ascites. Store at -20°C. Normal Mouse IgG. One vial containing 125 μg purified mouse IgG in 125 μL storage buffer containing 0.1% sodium azide. Store at -20°C. ChIP Primers GAPDH Coding Region. One vial containing 75 μL of 5 μM of each primer specific for the coding region of human GAPDH. Store at -20°C. FOR: GGC TCC CAC CTT TCT CAT CC REV: GGC CAT CCA CAG TCT TCT GG

应用
应用	This ChIPAb+ HP1γ -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
主要应用	Chromatin Immunoprecipitation (ChIP) Western Blotting
应用说明	Chromatin Immunoprecipitation: Representative lot data. Sonicated chromatin prepared from HeLa S3 cells (1 X 10⁶ cell equivalents per IP) was subjected to chromatin immunoprecipitation using 5 µg of either a normal mouse IgG or HP1γ antibody and the Magna ChIP G Kit (Cat. # 17-611). Successful immunoprecipitation of HP1γ associated DNA fragments was verified by qPCR using MYOD promoter (negative) and GAPDH coding (positive) (Please see figures). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated. Please refer to the EZ-Magna G ChIP™ (Cat. # 17-409) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.

生物信息
免疫原品种	HP1γ ascites is made against a GST fusion protein corresponding to amino acids 17-173 of human HP1γ.
表位	a.a. 17-173
宿主	Mouse
特异性	Recognizes HP1γ, MW: ~25 kDa.
同种型	IgG1
品种反应性	VertebratesHumanMouse
Species Reactivity Note	Human, mouse. Not tested in other species. The immunogen sequence is identical in a wide range of animal and plant species, so broad cross-reactivity is expected.
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_009207
Entrez基因汇总	At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene.
基因符号	HP1Hs-gamma CBX3 HECH HP1-GAMMA
UniProt编号	Q13185

产品使用声明
质量保证	Chromatin Immunoprecipitation: Sonicated chromatin prepared from HeLa S3 cells (1 X 10⁶ cell equivalents per IP) were subjected to chromatin immunoprecipitation using 5 µg of either a normal mouse IgG or HP1γ antibody and the Magna ChIP™ G Kit (Cat. # 17-611). Successful immunoprecipitation of HP1γ-associated DNA fragments was verified by qPCR using Control Primers (Please see figures). Please refer to the EZ-Magna ChIP™ G (Cat. # 17-409) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品使用声明

质量保证

Chromatin Immunoprecipitation:
Sonicated chromatin prepared from HeLa S3 cells (1 X 10⁶ cell equivalents per IP) were subjected to chromatin immunoprecipitation using 5 µg of either a normal mouse IgG or HP1γ antibody and the Magna ChIP™ G Kit (Cat. # 17-611). Successful immunoprecipitation of HP1γ-associated DNA fragments was verified by qPCR using Control Primers (Please see figures).
Please refer to the EZ-Magna ChIP™ G (Cat. # 17-409) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.