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merck millipore,默克密理博,17-604,ChIPAb+ LEF1 - ChIP Validated Antibody and Primer Set
产品名称：ChIPAb+ LEF1 - ChIP Validated Antibody and Primer Set
产品型号：17-604
Use ChIPAb+ LEF1 Mouse Monoclonal Antibody validated in WB, ChIP for the detection of LEF1.
merck millipore,默克密理博,17-604,ChIPAb+ LEF1 - ChIP Validated Antibody and Primer Set
- 产品介绍
重要规格表
品种反应性 主要应用 M, H ChIP, WB 描述 产品目录编号 17-604 品牌系列 Upstate
商名 - Upstate
- ChIPAb+
描述 ChIPAb+ LEF1 - ChIP Validated Antibody and Primer Set 概述 Every lot of the ChIPAb+ line of antibodies is individually validated for chromatin precipitation, in order to guarantee successful ChIP assays every time. Each antibody includes a control primer set for performance confirmation. LEF1 antibody and the negative control antibody (mouse normal IgG) can be used to demonstrate that the LEF1 antibody is functionally validated in the precipitation of LEF1 associated chromatin.
The qPCR primers included flank the LEF1/TCF binding site in human c-myc promoter.产品信息 格式 Purified 控制 - Includes negative control mouse IgG antibody and control primers specific for human c-myc promoter.
演示 Anti-LEF1 (mouse IgG1). 1 vial containing 100 mg of thiophilic and size exclusion chromatography purified mouse IgG1 in 100 mL of phosphate buffered saline with sodium azide. Store at-20°C. The antibody is made against amino acid residues 1-85 of human LEF1 and can recognize human and mouse LEF1. It does not cross-react with TCF-3 or TCF-4.
Normal Mouse IgG. One vial containing 125 μg of normal mouse IgG in 125 μL volume. Store at -20°C.
ChIP primers c-myc. One vial containing 75 mL of 5 μM of each control primer specific for human c-myc promoter. Store at -20°C.
FOR: CCC AAA AAA AGG CAC GGA A
REV: TAT TGG AAA TGC GGT CAT GC应用 应用 Use ChIPAb+ LEF1 Mouse Monoclonal Antibody validated in WB, ChIP for the detection of LEF1. 主要应用 - Chromatin Immunoprecipitation (ChIP)
- Western Blotting
应用说明 Western Blot Analysis:
Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-LEF1 (1 mg/mL). Proteins were visualized using a goat antimouse secondary antibody conjugated to HRP and a chemiluminescence detection system (Please see figures).生物信息 免疫原品种 The antibody is made against amino acid residues 1-85 of human LEF1. 宿主 Mouse 特异性 Recognizes LEF1 (lymphoid enhancer-binding factor 1). 同种型 IgG1 品种反应性 MouseHuman 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_016269.2
Entrez基因汇总 Lymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha (TCRA; MIM 186880) enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1 (HMG1; MIM 163905).[supplied by OMIM] 基因符号 - TCF1-alpha
- LEF1
- DKFZp586H0919
- TCF1ALPHA
- LEF-1
UniProt编号 - Q9UJU2
UniProt汇总 FUNCTION: SwissProt: Q9UJU2 # Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling.
SIZE: 399 amino acids; 44201 Da
SUBUNIT: Binds the armadillo repeat of CTNNB1 and forms a stable complex. Interacts with EP300, TLE1 and PIASG (By similarity). Binds THOC4, MDFI and MDFIC.
SUBCELLULAR LOCATION: Nucleus (By similarity). Note=Found in nuclear bodies upon PIASG binding (By similarity).
TISSUE SPECIFICITY: Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines.DOMAIN:SwissProt: Q9UJU2 Proline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors.
SIMILARITY: Belongs to the TCF/LEF family. & Contains 1 HMG box DNA-binding domain.产品使用声明 质量保证 Chromatin Immunoprecipitation:
Sonicated chromatin prepared from 3x106 HT29 cells was subjected to chromatin immunoprecipitation using 4 μg of either the negative control antibody, Mouse Normal IgG or mouse Anti-LEF1 and the Magna ChIP™ G kit (Cat.# 17-611).
Successful enrichment of LEF1 associated DNA fragments was verified by qPCR using ChIP Primers c-myc flanking the human c-myc promoter that contains LEF1 binding sites (Please see figures).
Please refer to the EZ-Magna ChIP™ G (Cat.# 17-409) or EZ-ChIP™ (Cat.# 17-371) kit protocols for experimental details.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 1 year at -20°C from date of shipment 包装信息 数量 25 assays 包装 25 assays per kit. ~4μg per chromatin immunoprecipitation.
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