merck millipore,默克密理博,17-306,Control Histones (+/- colcemid)

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merck millipore,默克密理博,17-306,Control Histones (+/- colcemid)
产品名称：Control Histones (+/- colcemid)
产品型号：17-306
Core histones, including histone H1, purified by acid extraction precipitation from log phase untreated & colcemid-treated HeLa cells.

merck millipore,默克密理博,17-306,Control Histones (+/- colcemid)

产品介绍

merck millipore,默克密理博,17-306,Control Histones (+/- colcemid)

重要规格表

主要应用	Entrez基因编号	Uni Prot编号
WB	NM_005321.2	P10412

描述
产品目录编号	17-306
品牌系列	Upstate
商名	Upstate
描述	Control Histones (+/- colcemid)
概述	Product Description: Core histones, including histone H1, purified by acid extraction precipitation from log phase untreated and colcemid-treated HeLa cells.

产品信息
部件	Control Histones, treated Control Histones, untreated

应用
应用	Core histones, including histone H1, purified by acid extraction precipitation from log phase untreated & colcemid-treated HeLa cells.
主要应用	Western Blotting

生物信息
品种	Human
Entrez基因编号	NM_005321.2
Entrez基因汇总	Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
基因符号	MGC116819 HIST1H1E H1F4 H1.4 dJ221C16.5
UniProt编号	P10412
UniProt汇总	FUNCTION: SwissProt: P10412 # Histones H1 are necessary for the condensation of nucleosome chains into higher order structures. SIZE: 219 amino acids; 21865 Da SUBCELLULAR LOCATION: Nucleus. SIMILARITY: SwissProt: P10412 ## Belongs to the histone H1/H5 family. MISCELLANEOUS: This variant accounts for 60% of histone H1.

产品使用声明
质量保证	Routinely evaluated by western blot. Anti-phospho-Histone H3 (Ser10) (06-570) or Anti-phospho-Histone H3 (Ser28) (07-145) were tested on histones from untreated and colcemid-treated HeLa cells.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Lyophilized: Stable for 2 years at -20°C from date of shipment, when stored with provided desiccant. Reconstituted: Stable for 6 months at -70°C. Rehydration: Aseptically reconstitute to 1 mg/mL with sterile water. Control Histones, untreated may be rehydrated in 2X RSB (reticulocyte standard buffer) if used exclusively for immunoblotting. Aliquot to avoid repeated freezing and thawing. RSB, 1X: 10 mM Tris-HCl, pH 7.6, 3 mM MgCl₂, 10 mM NaCl, 1 mM PMSF, and protein phosphatase inhibitors

储存和货运信息

存储条件

Lyophilized: Stable for 2 years at -20°C from date of shipment, when stored with provided desiccant.
Reconstituted: Stable for 6 months at -70°C.

Rehydration:
Aseptically reconstitute to 1 mg/mL with sterile water. Control Histones, untreated may be rehydrated in 2X RSB (reticulocyte standard buffer) if used exclusively for immunoblotting. Aliquot to avoid repeated freezing and thawing.
RSB, 1X: 10 mM Tris-HCl, pH 7.6, 3 mM MgCl₂, 10 mM NaCl, 1 mM PMSF, and protein phosphatase inhibitors