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品种反应性 主要应用 宿主 格式 抗体类型 Xn, Ca, H, M, Mk, R, Rb, Sh WB, ELISA, IHC Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB3184 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-MEK5 Antibody 产品信息 格式 Affinity Purified 演示 Affinity purified immunoglobulin. Affinity purified immunoglobulin
Liquid containing PBS, pH 7.2. No preservatives.应用 应用 Anti-MEK5 Antibody is an antibody against MEK5 for use in ELISA, IH, IP & WB. 主要应用 - Western Blotting
- ELISA
- Immunohistochemistry
应用说明 Western Blot: 1 μg/mL
Immunohistochemistry: 1:1,000
Immunoprecipitation
EIA
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Human MEK 5 synthetic peptide (aa. 59-74; Genbank Accession # U71088), conjugated to KLH; identical to rat MEK5 sequence over these residues. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 MEK 5 is also known as MAP Kinase Kinase 5, or ERK Kinase 5. AB3184 detects a 49 kDa protein, corresponding to the expeted molecular mass of Mek5, in rat brain, rat liver, rat kidney, rat heart, mouse brain, and mouse testis tissue extracts as well as in cell lysates prepared from the rat pheochromacytoma (PC12) and the human HeLa cell lines. No cross-reactivity with MEK 1, 2, 3, 4 or 6 has been observed with this antibody. 品种反应性 XenopusCanineHumanMouseMonkeyRatRabbitSheep 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_145160.1
- NM_002757.2
Entrez基因汇总 The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been described. 基因符号 - EC 2.7.12.2
- MAP2K5
- MAPKK5
- HsT17454
- PRKMK5
- MEK5
- MKK5
UniProt编号 - Q13163
UniProt汇总 FUNCTION: SwissProt: Q13163 # May participate together with ERK5 in a growth factor stimulated signaling cascade. Specifically activates ERK5, but not other MAP kinases such as ERK2 or p38. Is not phosphorylated by RAFA, RAFB or RAFC. May interact with GTPases such as CDC42.
SIZE: 448 amino acids; 50112 Da
SUBUNIT: Interacts with PARD6A and MAP3K3. Forms a complex with SQSTM1 and PRKCZ or PRKCI (By similarity). Interacts with Yersinia yopJ.
TISSUE SPECIFICITY: Expressed in many adult tissues. Abundant in heart and skeletal muscle.
DOMAIN:SwissProt: Q13163 The OPR domain mediates interactions with SQSTM1, PARD6A and MAP3K3 (By similarity).
PTM: Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases (By similarity). & Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.
SIMILARITY: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. & Contains 1 OPR domain. & Contains 1 protein kinase domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -20°C in undiluted aliquots for up to 12 months from date of receipt. Thawed product may be stored at 2-8°C for up to 4 weeks. Avoid repeated freeze/thaw cycles. 包装信息 数量 50 µg
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