merck millipore,默克密理博,17-10260,ChIPAb+™ N-CoR Antibody

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merck millipore,默克密理博,17-10260,ChIPAb+™ N-CoR Antibody
产品名称：ChIPAb+™ N-CoR Antibody
产品型号：17-10260
This ChIPAb+ N-CoR -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.

merck millipore,默克密理博,17-10260,ChIPAb+™ N-CoR Antibody

产品介绍

merck millipore,默克密理博,17-10260,ChIPAb+™ N-CoR Antibody

重要规格表

品种反应性	主要应用
M, H	ChIP, WB

描述
产品目录编号	17-10260
商名	Upstate ChIPAb+
描述	ChIPAb+™ N-CoR Antibody
概述	All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction. The ChIPAb+JMJD1C set includes the JMJD1C antibody, a Normal Rabbit IgG, and control primers which amplify a 110 bp region of ChIP Primers, human β-globin. The JMJD1C and negative controls are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of JMJD1C -associated chromatin.
Alternate Names	Retinoid X receptor-interacting protein 13 N-CoR1 N-CoR Nuclear receptor corepressor 1
背景信息	In the absence of ligands, the corepressor N-CoR represses nuclear hormone receptor-mediated gene transcription. In the presence of ligands, these hormone receptors activate gene expression and play critical roles in development, growth, and homeostasis. N-Cor-mediated transcriptional repression is facilitated through the formation of histone deacetylase (HDACs) N-CoR complexes or by conjugation from small ubiquitin-like modifier 1 (SUMO-1). It is shown that the SANT1 domain of N-CoR serves as a binding region for SUMO-E2 conjugating enzyme Ubc9 and SUMO-E3 ligase Pias1, which is necessary for N-CoR-mediated repression of respective reporter genes. SUMOylation-deficient N-COR knockout mice display a signification reduction in the repression of gene transcription and result in defects involving the differentiation and development of neural cells into erythrocytes and thymocytes.

产品信息
格式	Affinity Purified
控制	Includes normal rabbit IgG and primers specific for human p21.
演示	Components: Anti-N-CoR (Rabbit Polyclonal). One vial containing 75 µg of affinity purified polyclonal in buffer containing 0.1M Tris-glycine (pH 7.4) and 150 mM NaCl with 0.05% sodium azide before the addition of 30% glycerol. Store at -20°C. Concentration: 0.7 mg/mL Normal Rabbit IgG One vial containing 125 µg of Rabbit IgG in 125 µL of storage buffer containing 0.05% sodium azide. Store at -20°C. ChIP Primers, p21. One vial containing 75 μL of each primer (5 μM) specific for the human p21 (WAF1/CIP1/CDKN1A) promoter. Store at -20°C. FOR: CCC ACA GCA GAG GAG AAA GAA REV: CTG GAA ATC TCT GCC CAG ACA

应用
应用	This ChIPAb+ N-CoR -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
主要应用	Chromatin Immunoprecipitation (ChIP) Western Blotting
应用说明	Chromatin Immunoprecipitation: Representative lot data. Sonicated chromatin prepared from untreated or UV treated (6 hrs, 50 joules/m2.) U2OS cells (3 X 10E6 cell equivalents per IP) was subjected to chromatin immunoprecipitation using using 3 µg of either Normal Rabbit IgGor 3 µg of Anti-N-CoR and the Magna ChIP™ A/G Kit (Cat. # 17-10085). Successful immunoprecipitation of N-CoR associated DNA fragments was verified by qPCR using ChIP Primers, p21. (Figure 2). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated. Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details. Western Blot Analysis: Representative lot data. RAW 264.7 cell lysate was probed with Anti-N-CoR (1 µg/mL). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Anti-N-CoR (~270 kDa). (Figure 3).

生物信息
免疫原品种	KLH-conjugated linear peptide corresponding to a region between amino acids 1510 and 1540 of the N-CoR protein.
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
品种反应性	MouseHuman
Species Reactivity Note	Demonstrated to react with human and mouse.
抗体类型	Polyclonal Antibody
Entrez基因编号	NP_035438
基因符号	Rxrip13 Ncor1
纯化方法	Affinity purified
UniProt编号	Q60974.
UniProt汇总	FUNCTION: Mediates transcriptional repression by certain nuclear receptors. Part of a complex which promotes histone deacetylation and the formation of repressive chromatin structures which may impede the access of basal transcription factors. SUBUNIT STRUCTURE: Interacts with ZBTB33; the interaction serves to recruit the N-CoR complex to promoter regions containing methylated CpG dinucleotides. Interacts with TRIM28 and KDM3A. Interacts (via the RD1 domain) with BAZ1A (via its N-terminal); the interaction corepresses a number of NCOR1-regulated genes. Interacts with HEXIM1 By similarity. Interacts with C1D, SIAH2, HDAC7, SAP30, SIN3A and SIN3B. Forms a large corepressor complex that contains SIN3A/B and histone deacetylases HDAC1 and HDAC2. This complex associates with the thyroid receptor (TR) and the retinoid acid receptor (RAR) in the absence of ligand. Interacts directly with RARA; the interaction is facilitated with RARA trimethylation. Interacts with DACH1. Component of the N-Cor repressor complex, at least composed of CBFA2T3, HEXIM1, NCOR1, NCOR2, HDAC3, TBL1X, TBL1XR1, CORO2A and GPS2. SUBCELLULAR LOCATION: Nucleus.

产品使用声明
质量保证	Chromatin Immunoprecipitation: Representative lot data. Sonicated chromatin prepared from U2OS cells (3 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 3 µg of either Normal Rabbit IgGor 3 µg of Anti-N-CoR and the Magna ChIP™ A/G Kit (Cat. # 17-10085). Successful immunoprecipitation of N-CoR associated DNA fragments was verified by qPCR using ChIP Primers, p21flanking an Sp1 binding site in the human p21 promoter. (Figure 1). Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品使用声明

质量保证

Chromatin Immunoprecipitation:
Representative lot data.
Sonicated chromatin prepared from U2OS cells (3 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 3 µg of either Normal Rabbit IgGor 3 µg of Anti-N-CoR and the Magna ChIP™ A/G Kit (Cat. # 17-10085). Successful immunoprecipitation of N-CoR associated DNA fragments was verified by qPCR using ChIP Primers, p21flanking an Sp1 binding site in the human p21 promoter. (Figure 1).
Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.