- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
描述 产品目录编号 14-346M 品牌系列 Upstate
商名 - Upstate
描述 CHK1 Protein, active, 250 µg 概述 N-terminal GST tagged, human full length CHK1 应用 应用 Active, N-terminal GST tagged, human full length CHK1, for use in Kinase Assays. 主要应用 - Kinase Assay
生物信息 来源 expressed in SF21 cells 品种 Human 特异活性 For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. Entrez基因编号 - NM_001274.3
基因符号 - CHK1
- CHEK1
蛋白靶 CHK1 纯化方法 Glutathione-agarose 靶子系列 CAMK UniProt编号 - O14757
UniProt汇总 FUNCTION: SwissProt: O14757 # Required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Recognizes the substrate consensus sequence [R-X-X- S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser- 293' promotes proteolysis of CDC25A. Inhibition of CDC25 activity leads to increased inhibitory tyrosine phosphorylation of CDK- cyclin complexes and blocks cell cycle progression. Binds to and phosphorylates RAD51 at 'Thr-309', which may enhance the association of RAD51 with chromatin and promote DNA repair by homologous recombination. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may affect chromatin assembly during S phase or DNA repair. May also phosphorylate multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and enhances suppression of cellular proliferation.
SIZE: 476 amino acids; 54420 Da
SUBUNIT: Interacts with BRCA1, CLSPN, PPM1D, RAD51, TIMELESS, XPO1/CRM1 and YWHAZ/14-3-3 zeta.
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Nuclear export is mediated at least in part by XPO1/CRM1. Also localizes to the centrosome specifically during interphase, where it may protect centrosomal CDC2 kinase from inappropriate activation by cytoplasmic CDC25B.
TISSUE SPECIFICITY: Expressed ubiquitously with the most abundant expression in thymus, testis, small intestine and colon.
DOMAIN: SwissProt: O14757 The autoinhibitory region (AIR) inhibits the activity of the kinase domain.
PTM: Phosphorylated by ATR in a RAD17-dependent manner in response to ultraviolet irradiation and inhibition of DNA replication. Phosphorylated by ATM in response to ionizing irradiation. ATM and ATR can both phosphorylate Ser-317 and Ser-345 and this results in enhanced kinase activity. Phosphorylation at Ser-345 also increases binding to 14-3-3 proteins and promotes nuclear retention. Conversely, dephosphorylation at Ser-345 by PPM1D may contribute to exit from checkpoint mediated cell cycle arrest. May also be phosphorylated at Ser-280 by AKT1/PKB, which may promote mono and/or diubiquitination. Also phosphorylated at undefined residues during mitotic arrest, which results in decreased activity. & Ubiquitinated. Mono or diubiquitination promotes nuclear exclusion (By similarity).
SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. NIM1 subfamily. & Contains 1 protein kinase domain.产品使用声明 质量保证 routinely evaluated by phosphorylation of the CHKtide substrate peptide 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 1 year at -70C 包装信息 数量 250 µg 包装 Also available in 10 μg size (cat#14-346) and in bulk (cat# 14-346-K).
上一件merck millipore产品：merck millipore,默克密理博,HTMP1MAG-54K,MILLIPLEX MAP Human TIMP Magnetic Bead Panel 1 - Immunology Multiplex Assay
下一件merck millipore产品：merck millipore,默克密理博,06-1433,Anti-phospho-FGFR-1 (Tyr653/Tyr654) Antibody
相关产品
merck millipore,默克密理博,324674,EGFR Inhibitor - CAS 879127-07-8 - Calbiochem
merck millipore,默克密理博,71186,YeastBuster™ Protein Extraction Reagent
merck millipore,默克密理博,MAB3584,Anti-Heterochromatin Protein-1 α Antibody, clone 2HP-1H5
merck millipore,默克密理博,17-10260,ChIPAb+™ N-CoR Antibody
merck millipore,默克密理博,06-1433,Anti-phospho-FGFR-1 (Tyr653/Tyr654) Antibody
merck millipore,默克密理博,MAB5220,Anti-NMDAR2B Antibody, clone 13A11
merck millipore,默克密理博,ST1714,Anti-PAPPA Mouse mAb (1G3)
merck millipore,默克密理博,17-10264,ChIPAb+™ JHDM1B Antibody
merck millipore,默克密理博,FCMAB111F,Milli-Mark™ Anti-HESCA-1-FITC Antibody, clone 051007-4A5