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merck millipore,默克密理博,06-1433,Anti-phospho-FGFR-1 (Tyr653/Tyr654) Antibody
产品名称：Anti-phospho-FGFR-1 (Tyr653/Tyr654) Antibody
产品型号：06-1433
Detect phospho-FGFR-1 (Tyr653/Tyr654) using this Anti-phospho-FGFR-1 (Tyr653/Tyr654) Antibody validated for use in WB, Cell Function Assay.
merck millipore,默克密理博,06-1433,Anti-phospho-FGFR-1 (Tyr653/Tyr654) Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Xn, H, M, R, Ch, B WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 06-1433 描述 Anti-phospho-FGFR-1 (Tyr653/Tyr654) Antibody Alternate Names - CD331
- Basic fibroblast growth factor receptor 1
- FGFR-1
- bFGF-R-1
- Fms-like tyrosine kinase 2
- FLT-2
- Proto-oncogene c-Fgr
背景信息 FGFR-1 (fibroblast growth factor receptor 1) is a member of the basic fibroblast growth factor family of proteins and is thought to be important in the induction and propagation of benign prostatic hyperplasia. A shortage of FGFR-1 causes death post-implantation and has been linked to neurofascin and neurite outgrowth. FGFR-1 expression has recently been found to be increased in patients with renal cell carcinoma and FGFR-1 may also have a role to play in early limb formation. 产品信息 格式 Affinity Purified 控制 - Untreated and lambda phosphatase-treated HEK293T cells transfected with FGFR-1
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Detect phospho-FGFR-1 (Tyr653/Tyr654) using this Anti-phospho-FGFR-1 (Tyr653/Tyr654) Antibody validated for use in WB, Cell Function Assay. 主要应用 - Western Blotting
应用说明 Peptide Inhibition Assay Analysis: 0.2 µg/mL from a representative lot peptide blocked in HEK293T cells transfected with FGFR-1. 生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to human FGFR-1 phosphorylated at Tyr653/Tyr654. 表位 Phosphorylated Tyr653/Tyr654 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes FGFR-1 phosphorylated at Tyr653/Tyr654. 品种反应性 XenopusHumanMouseRatChickenBovine Species Reactivity Note Demonstrated to react with Human.
Predicted to react with Mouse, Rat, Chicken, Bovine, Xenopus, and Zebra Fish based on 100% sequence homology.抗体类型 Polyclonal Antibody Entrez基因编号 - NP_075598
Entrez基因汇总 The protein encoded by this gene is a member of the fibroblast growth factor receptor (FGFR) family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member binds both acidic and basic fibroblast growth factors and is involved in limb induction. Mutations in this gene have been associated with Pfeiffer syndrome, Jackson-Weiss syndrome, Antley-Bixler syndrome, osteoglophonic dysplasia, and autosomal dominant Kallmann syndrome 2. Chromosomal aberrations involving this gene are associated with stem cell myeloproliferative disorder and stem cell leukemia lymphoma syndrome. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq]. 基因符号 - FLT2
- FGFR1
- FGFBR
- FLG
修改 - Phosphorylation
纯化方法 Affinity Purified UniProt编号 - P11362
UniProt汇总 FUNCTION: Receptor for fibroblast growth factors FGF2 and FGF1. Receptor for FGF23 in the presence of KL (By similarity). Promotes mitogenesis in response to fibroblast growth factors. Activates PLCG1.
CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
SUBUNIT STRUCTURE: Homodimer. Interacts with KLB (By similarity). Interacts with KL and FGF23 (By similarity). Interacts with SHB and GRB10. Interacts with PLCG1 (via SH2 domains). Interacts with KAL1; this interaction does not interfere with FGF2-binding to FGFR1, but prevents binding of heparin-bound FGF2. Interacts with SOX2 and SOX3 (By similarity). SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Detected in astrocytoma, neuroblastoma and adrenal cortex cell lines. Some isoforms are detected in foreskin fibroblast cell lines, however isoform 17, isoform 18 and isoform 19 are not detected in these cells.
PTM: Binding of FGF1 and heparin promotes autophosphorylation on tyrosine residues and activation of the receptor.
INVOLVEMENT IN DISEASE: Defects in FGFR1 are a cause of Pfeiffer syndrome (PS) [MIM:101600]; also known as acrocephalosyndactyly type V (ACS5). PS is characterized by craniosynostosis (premature fusion of the skull sutures) with deviation and enlargement of the thumbs and great toes, brachymesophalangy, with phalangeal ankylosis and a varying degree of soft tissue syndactyly.
Defects in FGFR1 are a cause of idiopathic hypogonadotropic hypogonadism (IHH) [MIM:146110]. IHH is defined as a deficiency of the pituitary secretion of follicle-stimulating hormone and luteinizing hormone, which results in the impairment of pubertal maturation and of reproductive function.
Defects in FGFR1 are the cause of Kallmann syndrome type 2 (KAL2) [MIM:147950]; also known as hypogonadotropic hypogonadism and anosmia. Anosmia or hyposmia is related to the absence or hypoplasia of the olfactory bulbs and tracts. Hypogonadism is due to deficiency in gonadotropin-releasing hormone and probably results from a failure of embryonic migration of gonadotropin-releasing hormone-synthesizing neurons. In some cases, midline cranial anomalies (cleft lip/palate and imperfect fusion) are present and anosmia may be absent or inconspicuous.
Defects in FGFR1 are the cause of osteoglophonic dysplasia (OGD) [MIM:166250]; also known as osteoglophonic dwarfism. OGD is characterized by craniosynostosis, prominent supraorbital ridge, and depressed nasal bridge, as well as by rhizomelic dwarfism and nonossifying bone lesions. Inheritance is autosomal dominant.
Defects in FGFR1 are the cause of trigonocephaly non-syndromic (TRICEPH) [MIM:190440]; also known as metopic craniosynostosis. The term trigonocephaly describes the typical keel-shaped deformation of the forehead resulting from premature fusion of the frontal suture. Trigonocephaly may occur also as a part of a syndrome.
Note=A chromosomal aberration involving FGFR1 may be a cause of stem cell leukemia lymphoma syndrome (SCLL). Translocation t(8;13)(p11;q12) with ZMYM2. SCLL usually presents as lymphoblastic lymphoma in association with a myeloproliferative disorder, often accompanied by pronounced peripheral eosinophilia and/or prominent eosinophilic infiltrates in the affected bone marrow.
Note=A chromosomal aberration involving FGFR1 may be a cause of stem cell myeloproliferative disorder (MPD). Translocation t(6;8)(q27;p11) with FGFR1OP. Insertion ins(12;8)(p11;p11p22) with FGFR1OP2. MPD is characterized by myeloid hyperplasia, eosinophilia and T-cell or B-cell lymphoblastic lymphoma. In general it progresses to acute myeloid leukemia. The fusion proteins FGFR1OP2-FGFR1, FGFR1OP-FGFR1 or FGFR1-FGFR1OP may exhibit constitutive kinase activity and be responsible for the transforming activity.
Note=A chromosomal aberration involving FGFR1 may be a cause of stem cell myeloproliferative disorder (MPD). Translocation t(8;9)(p12;q33) with CEP110. MPD is characterized by myeloid hyperplasia, eosinophilia and T-cell or B-cell lymphoblastic lymphoma. In general it progresses to acute myeloid leukemia. The fusion protein CEP110-FGFR1 is found in the cytoplasm, exhibits constitutive kinase activity and may be responsible for the transforming activity.
SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain.产品使用声明 质量保证 Evaluated by Western Blot in untreated and lambda phosphatase-treated HEK293T cells transfected with FGFR1.
Western Blot Analysis: 0.2 µg/mL of this antibody detected FGFR-1 in 10 µg of untreated and lambda phosphatase-treated HEK293T cells transfected with FGFR1.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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