merck millipore,默克密理博,17-10051,ChIPAb+ Acetyl-Histone H3 (Lys14) - ChIP Validated Antibody and Primer Set, rabbit monoclonal

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merck millipore,默克密理博,17-10051,ChIPAb+ Acetyl-Histone H3 (Lys14) - ChIP Validated Antibody and Primer Set, rabbit monoclonal
产品名称：ChIPAb+ Acetyl-Histone H3 (Lys14) - ChIP Validated Antibody and Primer Set, rabbit monoclonal
产品型号：17-10051
This ChIPAb+ Acetyl-Histone H3 (Lys14) -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.

merck millipore,默克密理博,17-10051,ChIPAb+ Acetyl-Histone H3 (Lys14) - ChIP Validated Antibody and Primer Set, rabbit monoclonal

产品介绍

merck millipore,默克密理博,17-10051,ChIPAb+ Acetyl-Histone H3 (Lys14) - ChIP Validated Antibody and Primer Set, rabbit monoclonal

重要规格表

品种反应性	主要应用
R, H	ICC, ChIP, WB

描述
产品目录编号	17-10051
品牌系列	Upstate
商名	Upstate ChIPAb+
描述	ChIPAb+ Acetyl-Histone H3 (Lys14) - ChIP Validated Antibody and Primer Set, rabbit monoclonal
概述	All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction. The ChIPAb+ Acetyl-Histone H3 (Lys14) set includes the Acetyl-Histone H3 (Lys14) antibody, a negative control normal rabbit IgG, and qPCR primers which amplify a 89 bp region of human c-myc promoter. The Acetyl-Histone H3 (Lys14) and negative controls are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of Acetyl-Histone H3 (Lys14)-associated chromatin.
Alternate Names	H3 histone, family 3A H3K14Ac Histone H3 (acetyl K14)
背景信息	Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

产品信息
格式	Unpurified
控制	Includes negative control normal rabbit IgG and primers specific for human c-myc promoter.
演示	Anti-Acetyl-Histone H3 (Lys14) (rabbit monoclonal). One vial containing 50 µL of unpurified monoclonal antibody in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, with 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Store at -20°C. Nornal Rabbit IgG. One vial containing 75 μL of normal rabbit IgG. Store at -20°C. ChIP Primers, c-myc promoter. One vial containing 75 μL of 5 μM of each primer specific for human c-myc promoter. Store at -20°C. FOR: CCC AAA AAA AGG CAC GGA A REV: TAT TGG AAA TGC GGT CAT GC

应用
应用	This ChIPAb+ Acetyl-Histone H3 (Lys14) -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
主要应用	Immunocytochemistry Chromatin Immunoprecipitation (ChIP) Western Blotting
应用说明	Chromatin Immunoprecipitation: Representative lot data. Sonicated chromatin prepared from HeLa cells (1 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using either 2 µL of Normal rabbit IgG or 2 µL of Anti-acetyl-Histone H3 (Lys14) and the Magna ChIP™ A Kit (Cat. # 17-610). Successful immunoprecipitation of acetyl-Histone H3 (Lys14) associated DNA fragments was verified by qPCR using ChIP Primers c-myc as a positive locus, and myoD promoter primers as a negative locus. (Please see figures). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated. Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details. ChIP-seq Analysis: Chromatin immunoprecipitation was performed using the Magna ChIP™ HiSens kit (cat# 17-10460), 2 µL of anti-acetyl-Histone H3 (Lys14) antibody (cat# 17-10051), 20 µL Protein A/G beads, and 1e6 crosslinked HeLa cell chromatin followed by DNA purification using magnetic beads. Libraries were prepared from Input and ChIP DNA samples using standard protocols with Illumina barcoded adapters, and analyzed on Illumina HiSeq instrument. An excess of eighteen million reads from FastQ files were mapped using Bowtie following TagDust tag removal. Peaks were identified using MACS , with peaks and reads visualized as a custom track in UCSC Genome Browser from BigWig and BED files. Western Blot Analysis: Representative lot data. Sodium Butyrate-treated HeLa acid extract was resolved by electrophoresis, transferred to PVDF membrane and probed with Anti-Acetyl-Histone H3 (Lys14) (1:1000 dilution). To demonstrate specificity, serum was preincubated with modified Histone peptides: Lane 1: No Peptide Lane 2: Histone H3 Control Lane 3: Acetyl-Histone H3 (K9, K14) Lane 4: Acetyl Histone H3 (K9) Lane 5: Acetyl-Histone H3 (K14) Lane 6: Penta Acetyl Lys/Arg Lane 7: Acetyl-Histone H1 (K26) Lane 8: Acetyl-Histone H1.4 (K26) Lane 9: Histone H1.4 (acetyl K26/phospho K27) Lane 10: Trimethyl-Histone H3 (K4) Lane 11: Dimethyl-Histone H3 (K4) Lane 12: Monomethyl-Histone H3 (K4) Proteins were visualized using a donkey anti-rabbit IgG conjugated to HRP and visualized using a chemiluminescence detection system. Arrow indicates Histone H3 (~17 kDa) (Please see figures). Immunfluorescent IC Analysis: Representative lot data. A previous lot was used in immunofluorescent staining of HeLa cells using Anti-Acetyl-Histone H3 (Lys14) (Please see figures). Immunohistochemistry (Paraffin) Analysis: Representative lot data. A previous lot was used in immunohistochemical staining of paraffin-embedded human adenocarcinoma of uterus using Anti-Acetyl-Histone H3 (Lys14) (Please see figures).

生物信息
免疫原品种	Acetyl-specific synthetic peptide corres-ponding to residues around Lys14 of Histone H3.
表位	Acetylated Lys14
宿主	Rabbit
特异性	Recognizes Histone H3 acetylated on Lys14.
同种型	IgG
品种反应性	RatHuman
Species Reactivity Note	Human and rat.
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_003484.1
Entrez基因汇总	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
基因符号	OTTHUMP00000035621 H3.3A H3.3B H3F3 MGC87782 MGC87783 OTTHUMP00000035618 OTTHUMP00000035619
修改	Acetylation
纯化方法	Unpurified
UniProt编号	P84243
UniProt汇总	FUNCTION: Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. SUBUNIT STRUCTURE: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Interacts with HIRA, a chaperone required for its incorporation into nucleosomes. SUBCELLULAR LOCATION: Nucleus. DEVELOPMENTAL STAGE: Expressed throughout the cell cycle independently of DNA synthesis. PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8sme2). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me). Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription. Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8sme2) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters. Specifically enriched in modifications associated with active chromatin such as methylation at Lys-5 (H3K4me), Lys-37 and Lys-80. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me), which are linked to gene repression, are underrepresented. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin. Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, probably DAPK3. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation on Ser-32 is specific to regions bordering centromeres in metaphase chromosomes. Ubiquitinated By similarity. SEQUENCE SIMILARITIES: Belongs to the histone H3 family. SEQUENCE CAUTION: The sequence CAH73371.1 differs from that shown. Reason: Erroneous gene model prediction.

产品使用声明
质量保证	Chromatin Immunoprecipitation: Sonicated chromatin prepared from HeLa cells (1 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using either 2 µL of Normal Rabbit IgG or 2 µL of Anti-acetyl-Histone H3 (Lys14) and the Magna ChIP™ A Kit (Cat. # 17-610). Successful immunoprecipitation of acetyl-Histone H3 (Lys14) associated DNA fragments was verified by qPCR using ChIP Primers, c-myc (Please see figures). Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品使用声明

质量保证

Chromatin Immunoprecipitation:
Sonicated chromatin prepared from HeLa cells (1 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using either 2 µL of Normal Rabbit IgG or 2 µL of Anti-acetyl-Histone H3 (Lys14) and the Magna ChIP™ A Kit (Cat. # 17-610).
Successful immunoprecipitation of acetyl-Histone H3 (Lys14) associated DNA fragments was verified by qPCR using ChIP Primers, c-myc (Please see figures).

Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.