merck millipore,默克密理博,04-223,Anti-Cyclin E2 Antibody, clone EP454Y, rabbit monoclonal

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merck millipore,默克密理博,04-223,Anti-Cyclin E2 Antibody, clone EP454Y, rabbit monoclonal
产品名称：Anti-Cyclin E2 Antibody, clone EP454Y, rabbit monoclonal
产品型号：04-223
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-Cyclin E2 Antibody, clone EP454Y, rabbit detect More>>

merck millipore,默克密理博,04-223,Anti-Cyclin E2 Antibody, clone EP454Y, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-223,Anti-Cyclin E2 Antibody, clone EP454Y, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
H	ICC, WB	Rb	Culture Supernatant	Monoclonal Antibody

描述
产品目录编号	04-223
品牌系列	Upstate
商名	Upstate
描述	Anti-Cyclin E2 Antibody, clone EP454Y, rabbit monoclonal
Alternate Names	cyclin E2 G1/S-specific cyclin E2
背景信息	Cyclins are, as their name suggests, expressed at specific times during the cell cycle, and play a crucial role in the regulation of the cell cycle. Cyclins associate with cyclin-dependent kinases (cdks), and the association is absolutely required for cdk activity. At the conclusion of the relevant phase of the cell cycle, cyclins are rapidly ubiquitinated and degraded, thus returning cdks to their inactive state. Cyclin A associates with cdk2, and together with cyclin E is involved in the G1/S transition. Cyclin A/cdk2 remains active during S-phase. Cyclin B associates with cdk1/cdc2 to regulate the G2/M boundary. The D-cyclins bind cdk4 and cdk6, and result in Rb phosphorylation. The cyclin D1 gene was found in chronic B cell lymphocytic leukemia, and designated Bcl-1, though that nomenclature is no longer used.

产品信息
格式	Culture Supernatant
控制	Jurkat cell lysate.
演示	Unpurified rabbit monoclonal IgG in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-Cyclin E2 Antibody, clone EP454Y, rabbit detects level of Cyclin E2 & has been published & validated for use in IP, WB, IC, IH(P).
主要应用	Immunocytochemistry Western Blotting
应用说明	Immunohistochemistry: 1:250 – 500 dilution from a previous lot was used on immunohistochemistry. Immunoprecipitation: 1:50 dilution from a previous lot was used on immunocytochemistry. Immunocytochemistry: 1:100 – 250 dilution from a previous lot was used on immunocytochemistry.

生物信息
免疫原品种	KLH conjugated linear peptide.
表位	c-terminus
克隆	EP454Y
宿主	Rabbit
特异性	This antibody recognizes Cyclin E2.
同种型	IgG
品种反应性	Human
Species Reactivity Note	Proven to react with human. Not expected to react with rat and mouse.
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_477097.1
Entrez基因汇总	The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2. This cyclin has been shown to specifically interact with CIP/KIP family of CDK inhibitors, and plays a role in cell cycle G1/S transition. The expression of this gene peaks at the G1-S phase and exhibits a pattern of tissue specificity distinct from that of cyclin E1. A significantly increased expression level of this gene was observed in tumor-derived cells. [provided by RefSeq]
基因符号	CYCE2
UniProt编号	O96020
UniProt汇总	Function: Essential for the control of the cell cycle at the late G1 and early S phase. Subunit structure: Interacts with the CDK2 (in vivo) and CDK3 (in vitro) protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Subcellular location: Nucleus. Ref.3 Tissue specificity: According to Ref.1: highest levels in adult testis, thymus and brain. Lower levels in placenta, spleen and colon. Consistently elevated levels in tumor-derived cells compared to non-transformed proliferating cells. According to Ref.2: low levels in thymus, prostate, brain, skeletal muscle, and kidney. Elevated levels in lung. According to Ref.3: highly expressed in testis, placenta, thymus and brain. In a lesser extent in small intestine and colon. Induction: Activated by papilloma viral oncoproteins E6 and E7 which bind to and inactivate p53 and Rb, respectively. Post-translational modification: Phosphorylation by CDK2 triggers its release from CDK2 and degradation via the ubiquitin proteasome pathway By similarity. Sequence similarities: Belongs to the cyclin family. Cyclin E subfamily.

产品使用声明
质量保证	Routinely evaluated by western blot on Jurkat cell lysate. Western Blot Analysis: A 1:1,000-1:2,000 dilution of this lot was used to detect Cyclin E2 on Jurkat cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.