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品种反应性 主要应用 宿主 格式 抗体类型 H WB Rb Purified Polyclonal Antibody 描述 产品目录编号 06-413 品牌系列 Upstate
商名 - Upstate
描述 Anti-NFκB p52 Antibody 背景信息 The NFκB transcription factor was originally identified as a protein complex consisting of a 65 kDa DNA binding subunit and an associated 50 kDa protein. The 65 kDa subunit is functionally related to c-Rel p75 and Rel B p68. The p50 subunit was initially believed to be a functionally unique protein derived from the amino terminus of a precursor designated p105. A cDNA has been isolated that encodes an alternative DNA binding subunit of NFκB. It is synthesized as a 100 kDa protein that is expressed in a variety of cell types and, like p105, undergoes cleavage to generate its NFκB subunit, in this case a protein designated p52 (previously referred to as p49). In contrast to p50 derived from p105, p52 acts in synergy with p65 to stimulate the HIV enhancer in transiently transfected Jurkat cells. 产品信息 格式 Purified 控制 - Raji cell lysate, Jurkat whole cell lysate
演示 Protein A Purified immunoglobulin in in 0.1M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% NaN3 as a preservative. 应用 应用 Detect NFκB p52 with Anti-NFκB p52 Antibody (Rabbit Polyclonal Antibody), that has been shown to work in IP & WB. 主要应用 - Western Blotting
生物信息 免疫原品种 KLH conjugated synthetic peptide (CYNPGLDGIIEYDDFK) contained within amino acids 4-19 of human NF κB, p52 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes NFκB p52 and its precursor. 同种型 IgG 品种反应性 Human 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_019408
Entrez基因汇总 NFKB has been detected in numerous cell types that express cytokines, chemokines, growth factors, cell adhesion molecules, and some acute phase proteins in health and in various disease states. NFKB is activated by a wide variety of stimuli such as cytokines, oxidant-free radicals, inhaled particles, ultraviolet irradiation, and bacterial or viral products. Inappropriate activation of NF-kappa-B has been linked to inflammatory events associated with autoimmune arthritis, asthma, septic shock, lung fibrosis, glomerulonephritis, atherosclerosis, and AIDS. In contrast, complete and persistent inhibition of NF-kappa-B has been linked directly to apoptosis, inappropriate immune cell development, and delayed cell growth. For reviews, see Chen et al. (1999) [PubMed 9895331] and Baldwin (1996) [PubMed 8717528].[supplied by OMIM] 基因符号 - Lyt10
- NFKB2
- LYT10
- LYT-10
- H2TF1
纯化方法 Unpurified UniProt编号 - Q00653
UniProt汇总 FUNCTION: SwissProt: Q00653 # Appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. p52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. p52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65.
SIZE: 900 amino acids; 96749 Da
SUBUNIT: Active NF-kappa-B is a heterodimer of an about 52 kDa DNA-binding subunit and the weak DNA-binding subunit p65. Two heterodimers might form a labile tetramer.
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B).
DOMAIN: SwissProt: Q00653 The C-terminus of p100 might be involved in cytoplasmic retention, inhibition of DNA-binding by p52 homodimers, and/or transcription activation (By similarity). & The glycine-rich region (GRR) appears to be a critical element in the generation of p52.
PTM: While translation occurs, the particular unfolded structure after the GRR repeat promotes the generation of p52 making it an acceptable substrate for the proteasome. This process is known as cotranslational processing. The processed form is active and the unprocessed form acts as an inhibitor (I kappa B-like), being able to form cytosolic complexes with NF-kappa B, trapping it in the cytoplasm. Complete folding of the region downstream of the GRR repeat precludes processing. & Subsequent to MAP3K14-dependent serine phosphorylation, p100 polyubiquitination occurs then triggering its proteasome-dependent processing. & Constitutive processing is tightly suppressed by its C- terminal processing inhibitory domain, named PID, which contains the death domain.
DISEASE: SwissProt: Q00653 # A chromosomal aberration involving NFKB2 is found in a case of B-cell non Hodgkin lymphoma (B-NHL). Translocation t(10;14)(q24;q32) with IGHA1. The resulting oncogene is also called Lyt-10C alpha variant. & A chromosomal aberration involving NFKB2 is found in a cutaneous T-cell leukemia (C-TCL) cell line. This rearrangement produces the p80HT gene which encodes for a truncated 80 kDa protein (p80HT). & In B-cell leukemia (B-CLL) cell line, LB40 and EB308, can be found after heterogeneous chromosomal aberrations, such as internal deletions.
SIMILARITY: Contains 7 ANK repeats. & Contains 1 death domain. & Contains 1 RHD (Rel-like) domain.产品使用声明 质量保证 routinely evaluated by immunoblot on RIPA lysates from Raji cells 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 包装信息 数量 100 µL
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