merck millipore,默克密理博,GF142,Platelet Derived Growth Factor-AA Protein, Recombinant human

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merck millipore,默克密理博,GF142,Platelet Derived Growth Factor-AA Protein, Recombinant human
产品名称：Platelet Derived Growth Factor-AA Protein, Recombinant human
产品型号：GF142
The Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle & connective tissue.

merck millipore,默克密理博,GF142,Platelet Derived Growth Factor-AA Protein, Recombinant human

产品介绍

merck millipore,默克密理博,GF142,Platelet Derived Growth Factor-AA Protein, Recombinant human

重要规格表

主要应用	品种	来源	纯度
CULT	Human	E.coli	Greater than 98% by SDS-PAGE and HPLC analysis.

描述
产品目录编号	GF142
Replaces	GF017
描述	Platelet Derived Growth Factor-AA Protein, Recombinant human
概述	Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle and connective tissue. PDGF, which is composed of a dimer of two chains termed the A chain and B chain, can be present as AA or BB homodimers or as an AB heterodimer. Human PDGF-AA is a 28.5 kDa A chain homodimer protein containing 250 amino acid residues.
Alternate Names	PDGF-AA

产品信息
演示	Lyophilized from a 10mM Acetic Acid solution.

应用
应用	The Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle & connective tissue.
主要应用	Cell Culture
应用说明	For most in vitro applications, PDGF-AA exerts its biological activity in the concentration range of 0.2 to 10.0 ng/mL. Responding cells are (partial list): Fibroblasts, connective tissue.

生物信息
纯度	Greater than 98% by SDS-PAGE and HPLC analysis.
来源	E.coli
品种	Human
特异活性	Human PDGF-AA is fully biologically active when compared to standards, as determined by colorimetric assay using BALB / 3T3 cells.
Entrez基因编号	NM_033023.3 NM_002607.4
Entrez基因汇总	The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer or as a heterodimer with the platelet-derived growth factor beta polypeptide, where the dimers are connected by disulfide bonds. Studies using knockout mice have shown cellular defects in oligodendrocytes, alveolar smooth muscle cells, and Leydig cells in the testis; knockout mice die either as embryos or shortly after birth. Two splice variants have been identified for this gene.
基因符号	PDGF1 PDGFA PDGF-1 PDGF-A
UniProt编号	P04085
UniProt汇总	FUNCTION: SwissProt: P04085 # Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. Binding of this growth factor to its affinity receptor elicits a variety of cellular responses. It is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound. SIZE: 211 amino acids; 24043 Da SUBUNIT: Antiparallel disulfide-linked dimer of non-identical (A and B) chains. Homodimers of A and B chains are implicated in transformation processes. Interacts with CSPG4. SUBCELLULAR LOCATION: Secreted. DOMAIN: SwissProt: P04085 The long form contains a basic insert which acts as a cell retention signal.SIMILARITY: Belongs to the PDGF/VEGF growth factor family. MISCELLANEOUS: A-A and B-B, as well as A-B, dimers can bind to the PDGF receptor.

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	The lyophilized PDGF-AA is stable for a few weeks at room temperature, but best stored at -80°C. After a quick spin, reconstitute in 10mM Acetic Acid to a concentration of 0.1-1.0 mg/mL. This solution can be diluted into aqueous buffered solutions and stored at 2-8°C for one week or -20°C for future use. Reconstituted PDGF-AA should be stored in undiluted aliquots at -20°C for up to six months.

储存和货运信息

存储条件

The lyophilized PDGF-AA is stable for a few weeks at room temperature, but best stored at -80°C. After a quick spin, reconstitute in 10mM Acetic Acid to a concentration of 0.1-1.0 mg/mL. This solution can be diluted into aqueous buffered solutions and stored at 2-8°C for one week or -20°C for future use. Reconstituted PDGF-AA should be stored in undiluted aliquots at -20°C for up to six months.