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品种反应性 主要应用 宿主 格式 抗体类型 R, H, M, Mk ICC, WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB3843 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Ezrin/Radixin/Moesin Antibody 产品信息 格式 Affinity Purified 演示 Protein A and peptide affinity purified immunoglobulin. Liquid in 10 mM sodium HEPES, pH 7.5, 150 mM NaCl, 100 μg/mL BSA, with 50% glycerol. 应用 应用 Detect Ezrin/Radixin/Moesin using this Anti-Ezrin/Radixin/Moesin Antibody validated for use in WB, IC, IH(P). 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Western blotting: 1:1,000. The 75 kDa band is Moesin and the 80kDa band is Ezrin and Radixin. (For best results, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight)
Immunohistochemistry (Paraffin): 1:50. Positive control tissue is breast carcinoma. Cellular localization is membrane and cytoplasm
Immunocytochemistry (IF): 1:100
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 KLH-conjugated, synthetic peptide corresponding to residues surrounding Thr567 of human ezrin. 宿主 Rabbit 特异性 Recognizes Ezrin/Radixin/Moesin. These proteins function as linkers between the plasma membrane and the actin cytoskeleton, and are involved in cell adhesion, membrane ruffling, and microvilli formation. This antibody does not cross-react with other related proteins such as merlin or band 4.1. 品种反应性 RatHumanMouseMonkey 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_004252.2
Entrez基因汇总 Members of the ezrin (VIL2; MIM 123900)-radixin (RDX; MIM 179410)-moesin (MSN; MIM 309845) (ERM) protein family are highly concentrated in the apical aspect of polarized epithelial cells. These cells are studded with microvilli containing bundles of actin filaments, which must attach to the membrane to assemble and maintain the microvilli. The ERM proteins, together with merlin, the NF2 (MIM 607379) gene product, are thought to be linkers between integral membrane and cytoskeletal proteins, and they bind directly to actin in vitro. Actin cytoskeleton reorganization requires the activation of a sodium/hydrogen exchanger (SLC9A3; MIM 182307). SLC9A3R1 is an ERM-binding protein.[supplied by OMIM] 基因符号 - EBP50
- SLC9A3R1
- +exchanger)
- NHERF1
- +H
- NHERF
- Na
- NHERF-1
UniProt编号 - O14745
UniProt汇总 FUNCTION: SwissProt: O14745 # Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling.
SIZE: 358 amino acids; 38868 Da
SUBUNIT: Homodimer, and heterodimer with SLC9A3R2. Binds the N- termini of VIL2, RDX and MSN. Binds the C-termini of PDGFRA, PDGFRB, ADRB2, NOS2 and CFTR. Binds ARHGAP17, EPI64, GNB2L1, OPRK1, GNAQ, CTNNB1 and PLCB3. Binds PDZK1 (By similarity). Interacts with CLCN3. Binds the C-terminus of PAG1. In resting T- cells, part of a PAG1-SLC9A3R1-MSN complex which is disrupted upon TCR activation. Forms a complex with CFTR and SLC4A7. Forms a complex with SLC4A7 and ATP6V1B1.
SUBCELLULAR LOCATION: Intracytoplasmic membrane; Peripheral membrane protein. Cell projection, filopodium. Cell projection, ruffle. Cell projection, microvillus. Note=Colocalizes with actin in microvilli-rich apical regions of the syncytiotrophoblast. Found in microvilli, ruffling membrane and filopodia of HeLa cells. Present in lipid rafts of T-cells.
TISSUE SPECIFICITY: Detected in liver, kidney, pancreas, prostate, spleen, small intestine and placenta, in particular in the syncytiotrophoblast.
PTM: Phosphorylated on serine residues.
SIMILARITY: SwissProt: O14745 ## Contains 2 PDZ (DHR) domains.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20ºC for 12 months, do not aliquot. 包装信息 数量 100 µL
上一件merck millipore产品：merck millipore,默克密理博,MAB3262B,Anti-BrdU Antibody, clone PRB-1, biotin conjugated
下一件merck millipore产品：merck millipore,默克密理博,MAB1501,Anti-Actin Antibody, clone C4
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