merck millipore,默克密理博,235432,Caspase-3 Intracellular Activity Assay Kit II (PhiPhiLux G₂D₂)

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merck millipore,默克密理博,235432,Caspase-3 Intracellular Activity Assay Kit II (PhiPhiLux G₂D₂)
产品名称：Caspase-3 Intracellular Activity Assay Kit II (PhiPhiLux G₂D₂)
产品型号：235432

merck millipore,默克密理博,235432,Caspase-3 Intracellular Activity Assay Kit II (PhiPhiLux G₂D₂)

产品介绍

merck millipore,默克密理博,235432,Caspase-3 Intracellular Activity Assay Kit II (PhiPhiLux G₂D₂)

重要规格表

检测方法
Fluorescence

描述
概述	Assay kit for the determination by flow cytometry or fluorescence microscopy of caspase-3-like activity in intact cells undergoing apoptosis. Can be used to evaluate the ability of a test substance or drug candidate to activate or inhibit caspase-3. The assay is based on the reaction of the enzyme with the cell-permeable PhiPhiLux^® G₂D₂ substrate. Each substrate molecule contains two fluorophores and the cleaved substrate fluoresces red.
产品目录编号	235432
品牌系列	Calbiochem®

参考
参考	Chang, S.H., et al. 2002. Exp. Cell Res. 277, 15. Kottke, T.J., et al. 2002. J. Biol. Chem. 277, 804. Liu, L., et al. 2002. Nat. Med. 8, 185. Telford, W.G., et al. 2002. Cytometry 47, 81. Packard, B.Z., et al. 2001. J. Immunol. 167, 5061. Finucane, D.M., et al. 1999. J. Biol. Chem. 274, 2225. Porter, A.G. and Janicke, R.U. 1999. Cell Death Differ. 6, 99. Robles, R., et al. 1999. Endocrinology 140, 2641. Hirata, H., et al. 1998. J. Exp. Med. 187, 587. Packard, B.Z., et al. 1998. J. Phys. Chem. B 102, 1820. Siegel, R.M., et al. 1998. J. Cell Biol. 141, 1243. Packard, B.Z., et al. 1997. Methods Enzymol. 278, 15. Nicholson, D.W., et al. 1995. Nature 376, 37.

参考

Chang, S.H., et al. 2002. Exp. Cell Res. 277, 15.
Kottke, T.J., et al. 2002. J. Biol. Chem. 277, 804.
Liu, L., et al. 2002. Nat. Med. 8, 185.
Telford, W.G., et al. 2002. Cytometry 47, 81.
Packard, B.Z., et al. 2001. J. Immunol. 167, 5061.
Finucane, D.M., et al. 1999. J. Biol. Chem. 274, 2225.
Porter, A.G. and Janicke, R.U. 1999. Cell Death Differ. 6, 99.
Robles, R., et al. 1999. Endocrinology 140, 2641.
Hirata, H., et al. 1998. J. Exp. Med. 187, 587.
Packard, B.Z., et al. 1998. J. Phys. Chem. B 102, 1820.
Siegel, R.M., et al. 1998. J. Cell Biol. 141, 1243.
Packard, B.Z., et al. 1997. Methods Enzymol. 278, 15.
Nicholson, D.W., et al. 1995. Nature 376, 37.

产品信息
检测方法	Fluorescence
声明	Sold under license of U.S. Patents 5,605,809 and 5,714,342. Not available for sale in Japan.
形式	30 Tests
格式	Flow cytometry or fluorescence microscopy
试剂盒包含	Flow Cytometry Buffer, PhiPhiLux^® G₂D₂ Substrate, and a user protocol.

生物信息
化验时间	1.5-2 h
样品类型	Intact cells

理化信息
最大排放	~580 nm
最大激励	~552 nm

产品使用声明
预定用途	The Intracellular Caspase-3 Assay Kit allows is intended for studying the activation of caspase-3 and subsequent downstream events in whole cells. This kit can be used for either flow cytometry or fluorescence microscopy.

储存和货运信息
货运代码	Dry Ice Only
毒性	Standard Handling
储存	-20°C
存储条件	Prior to storage, centrifuge the reagent-containing vials briefly to remove any liquid from caps. Store kit at -20°C. Upon thawing, shake until the solution appears homogeneous. AVOID FREEZE / THAW CYCLES OF SOLUTIONS.
避免冻结 / 解冻	Avoid freeze/thaw
无需冷冻	No