merck millipore,默克密理博,17-10006,Nitrotyrosine ELISA

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merck millipore,默克密理博,17-10006,Nitrotyrosine ELISA
产品名称：Nitrotyrosine ELISA
产品型号：17-10006
This Nitrotyrosine ELISA is used to measure & quantify Nitrotyrosine levels in Neuroscience & Toxicity research.

merck millipore,默克密理博,17-10006,Nitrotyrosine ELISA

产品介绍

merck millipore,默克密理博,17-10006,Nitrotyrosine ELISA

重要规格表

可用的分析物	品种反应性	主要应用	检测方法
Nitrotyrosine	Ca, M, H, R, Po, Eq, Rb, Gp, Ht, NhP	ELISA	Chemiluminescence

描述
产品目录编号	17-10006
品牌系列	Upstate
商名	Upstate
描述	Nitrotyrosine ELISA
概述	Oxidative stress has been implicated in the pathogenesis of neurodegenerative disorders, cancer, and aging. Oxidative stress occurs as a response to increased oxidants, decreased anti-oxidants, or failure to repair oxidative damage induced by reactive oxygen species (ROS). ROS are free radicals, reactive molecules containing oxygen, or molecules containing oxygen that generate free radicals. ROS include nitric oxide (NO), superoxide (O₂^-), peroxynitrite (ONOO^-), and hydroxyl radical (OH^-). ROS are cleared from cells by the action of superoxide dismutase (SOD), catalase and peroxidases. NO, produced by nitric oxide synthases (NOS), is a critical signaling molecule involved in many physiological processes including muscle contraction, vasodilation, neuronal transmission, and immune responses. Some of these effects are mediated through reaction with and activation of guanylate cyclase (GC), resulting in increased cGMP production. With a half-life of seconds, NO readily diffuses through the cytosol and cell membrane, allowing it to act inside or adjacent to the cell in which it was produced. ROS may interact to form agents that modify cellular proteins. Superoxide, produced by NADPH oxidase (phox), reacts with NO to form peroxynitrite. Intracellular peroxynitrite can modify proteins by interacting with and nitrating tyrosine residues to form 3-nitrotyrosine. Tyrosine nitration may increase (e.g. sGC, Src, PI3K, Akt), decrease (e.g. Mn-SOD, Ca⁺⁺-ATPase), or have no discernable effect (e.g. p53, VASP, α-Synuclein) on the activity of a particular protein. Upstate's new Nitrotyrosine Assay Kit, Chemiluminescence Detection (Cat. # 17-10006) is a competitive ELISA for the quantitation of tyrosine nitration. The kit includes all reagents needed for measurement of tyrosine nitration, including a pre-coated 96-well plates, Nitrotyrosine peptide standard, a Nitrotyrosine antibody, LumiGLO® chemiluminescent detection substrate, and wash buffers. The assay has a wide dynamic range and high precision, making this assay a valuable new tool for the study of tyrosine nitration.
背景信息	Oxidative stress has been implicated in the pathogenesis of neurodegenerative disorders, cancer and aging. Oxidative stress occurs as a response to increased oxidants, decreased antioxidants, or failure to repair oxidative damage induced by reactive oxygen species (ROS). ROS are free radicals, reactive molecules containing oxygen, or molecules containing oxygen that generate free radicals. ROS include nitric oxide (NO), superoxide (O2-), peroxynitrite (ONOO-), and hydroxyl radical (OH-). ROS are cleared from cells by the action of superoxide dismutase (SOD), catalase and peroxidases. NO, produced by nitric oxide synthases (NOS), is a critical signaling molecule involved in many physiological processes including muscle contraction, vasodilation, neuronal transmission, and immune responses. Some of these effects are mediated through reaction with and activation of guanylate cyclase (GC), resulting in increased cGMP production. With a half-life of seconds, NO readily diffuses through the cytosol and cell membrane, allowing it to act inside or adjacent to the cell in which it was produced. ROS may interact to form agents that modify cellular proteins. Superoxide, produced by NADPH oxidase (phox), reacts with NO to form peroxynitrite. Intracellular peroxynitrite can modify proteins by interacting with and nitrating tyrosine residues to form 3-nitrotyrosine. Tyrosine nitration may increase (e.g. sGC, Src, PI3K, Akt), decrease (e.g. Mn-SOD, Ca++-ATPase), or have no discernable effect (e.g. p53, VASP, α-Synuclein) on the activity of a particular protein.

产品信息
部件	12. 30% BSA: (Part No. CS205797) One vial containing 1mL of 30% BSA. 1. Streptavidin Capture Plate: (Part No. CS204971) One pre-coated 96-stripwell immunoplate sealed in a foil pouch. 2. U-Bottom Plate: (Part No. CS205781) One clear non-binding U-bottom plate. 3. TBS, 20X: (Part No. 20-190) One bottle (50 mL) of 50X TBS. 4. 20% Tween® 20: (Part No. 20-246) One bottle (3 mL) of 20% Tween® 20 (v/v). 5. LumiGlo Chemiluminescent Substrate Reagent A: (Part No. 20-212C) One bottle (10 mL) of substrate. 6. LumiGlo Chemiluminescent Substrate Reagent B: (Part No. 20-212D) One bottle (10 mL) of substrate. 7. Plate Covers: Two plate covers. 8. Nitrotyrosine Peptide Standard: (Part No. CS204980). One vial containing (200 µL) of Nitrotyrosine Peptide Standard Stock at 4.8mM. 9. 1000X Biotinylated Nitrotyrosine Peptide: (Part No. CS204990). One vial containing (15 µL) of Biotinylated Nitortyrosine Peptide Stock at 450µM. 10. 2000X Anti-Nitrotyrosine Detection Antibody: (Part No. CS204982) One vial (10 µL) of 2000x anti-Nitrotyrosine detection antibody. 11. Gt X Rb, HRP Secondary Antibody: (Part No. 90276) One vial (30 µL) of 2000X goat anti-rabbit HRP conjugate.
检测方法	Chemiluminescence

产品信息

部件

12. 30% BSA: (Part No. CS205797) One vial containing 1mL of 30% BSA.
1. Streptavidin Capture Plate: (Part No. CS204971) One pre-coated 96-stripwell immunoplate sealed in a foil pouch.
2. U-Bottom Plate: (Part No. CS205781) One clear non-binding U-bottom plate.
3. TBS, 20X: (Part No. 20-190) One bottle (50 mL) of 50X TBS.
4. 20% Tween® 20: (Part No. 20-246) One bottle (3 mL) of 20% Tween® 20 (v/v).
5. LumiGlo Chemiluminescent Substrate Reagent A: (Part No. 20-212C) One bottle (10 mL) of substrate.
6. LumiGlo Chemiluminescent Substrate Reagent B: (Part No. 20-212D) One bottle (10 mL) of substrate.
7. Plate Covers: Two plate covers.
8. Nitrotyrosine Peptide Standard: (Part No. CS204980). One vial containing (200 µL) of Nitrotyrosine Peptide Standard Stock at 4.8mM.
9. 1000X Biotinylated Nitrotyrosine Peptide: (Part No. CS204990). One vial containing (15 µL) of Biotinylated Nitortyrosine Peptide Stock at 450µM.
10. 2000X Anti-Nitrotyrosine Detection Antibody: (Part No. CS204982) One vial (10 µL) of 2000x anti-Nitrotyrosine detection antibody.
11. Gt X Rb, HRP Secondary Antibody: (Part No. 90276) One vial (30 µL) of 2000X goat anti-rabbit HRP conjugate.

检测方法

Chemiluminescence

应用
应用	This Nitrotyrosine ELISA is used to measure & quantify Nitrotyrosine levels in Neuroscience & Toxicity research.
主要应用	ELISA

生物信息
品种反应性	CanineMouseHumanRatPigHorseRabbitGuinea PigHamsterNon-human Primate
可用的分析物	Nitrotyrosine

理化信息
灵敏度	0.1 μM
标准曲线范围	0.1 μM - 1600 μM

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Maintain the unopened kit (17-10006-1) at 2-8ºC until expiration date. Maintain the unopened kit (17-10006-2) at -20ºC until expiration date. Avoid repeated freeze/thaw cycles, aliquot if necessary.