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品种反应性 主要应用 宿主 格式 抗体类型 R, M WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 07-2275 描述 Anti-phospho GSK3β Antibody (Ser389) Alternate Names - GSK-3 beta
- glycogen synthase kinase 3 beta
- GSK3beta isoform
背景信息 GSK3 (Glycogen Synthase Kinase 3) differs from most serine/threonine kinases in that it is active in the absence of the action of signaling pathways. Two isoforms exist, GSK3α and GSK3β. The function of GSK3 is to phosphorylate Glycogen Synthase and thereby inactivate it. Insulin action stimulates the PI3 Kinase pathway, resulting in Akt activation, which phosphorylates and inactivates GSK3. Glycogen Synthase is then rapidly dephosphorylated and activated. Other GSK3 substrates include Jun (on inhibitory sites), and eIF2B. Phosphorylation of Tau by GSK3 may relate to development of Alzheimer's disease. Phosphorylation state-specific antibodies directed against the Akt site (Ser21) on GSK3 are suitable for surrogate assays of the activation state of the pathway. 产品信息 格式 Affinity Purified 控制 - Mouse brain tissue lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. 应用 应用 This phospho GSK3β antibody is validated for use in WB for the detection of the phospho GSK3β protein. 主要应用 - Western Blotting
生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to mouse GSK3β. 表位 Phosphorylated Ser389 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes phosphorylated GSK3β (Ser389). 同种型 IgG 品种反应性 RatMouse Species Reactivity Note Demonstrated to react with mouse and rat.
抗体类型 Polyclonal Antibody Entrez基因编号 - NP_062801
基因符号 - GSK3B
纯化方法 Affinity Purfied UniProt编号 - Q9WV60
UniProt汇总 FUNCTION: Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. May phosphorylate MUC1 and decrease the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin by similarity.
CATALYTIC ACTIVITY: ATP + [tau protein] = ADP + [tau protein] phosphate.
SUBUNIT STRUCTURE: Monomer By similarity. Interacts with AXIN1, CABYR, MMP2, MUC1, NIN and PRUNE By similarity. Interacts with ARRB2.
PTM: Phosphorylation on Tyr-216 is necessary for the activity By similarity.
SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
Contains 1 protein kinase domain.产品使用声明 质量保证 Evaluated by Western Blot in mouse brain tissue lysate.
Western Blot Analysis: 1:500 dilution of this antibody detected GSK3β on 10 µg of mouse brain tissue lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 200 µL
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