merck millipore,默克密理博,16-101,Anti-Phosphotyrosine Antibody, clone 4G10, agarose conjugate

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Merck Millipore 默克密理愽

品种反应性	主要应用	宿主	格式	抗体类型
Vrt	IP	M	Gel Immobilized	Monoclonal Antibody

描述
产品目录编号	16-101
品牌系列	Upstate
商名	Upstate
描述	Anti-Phosphotyrosine Antibody, clone 4G10®, agarose conjugate
概述	Anti-Phosphotyrosine antibody (05-321), cross-linked to protein A agarose by dimethylpimelimidate
背景信息	Some of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine). Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. The advent of anti-phospho-tyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.

产品信息
格式	Gel Immobilized
控制	Pervanadate-treated human A431 cell extracts, EGF-treated human A431 cells.
演示	1 mg of Anti-Phosphotyrosine monoclonal antibody covalently coupled to 1 mL of protein A agarose beads and provided as a 50% slurry suspended in PBS, containing 0.05% ProClin^® 150 as a preservative, for a total volume of 2 mL. Liquid suspension.

应用
应用	Anti-Phosphotyrosine Antibody, clone 4G10®, agarose conjugate is an antibody against Phosphotyrosine for use in IP.
主要应用	Immunoprecipitation
应用说明	This product is useful for immunoprecipitation and immunoaffinity chromatography methods. Suitable for immunoprecipitation and affinity purification.

生物信息
免疫原品种	Phosphotyramine-KLH.
克隆	4G10®,
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Mouse
特异性	Recognizes tyrosine-phosphorylated proteins from all species.
品种反应性	Vertebrates
Species Reactivity Note	All
抗体类型	Monoclonal Antibody
修改	Phosphorylation
纯化方法	Protein A purfied

产品使用声明
质量保证	Evaluated by immunoprecipitating the EGF receptor and other tyrosine phosphorylated proteins from EGF-stimulated A431 cells. Immunoprecipitation Analysis: 10-50 μL of this antibody immunoprecipitated the EGF receptor and other tyrosine phosphorylated proteins from EGFstimulated A431 cells.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at 2-8ºC from date of receipt.

包装信息
数量	1 mg

merck millipore,默克密理博,16-101,Anti-Phosphotyrosine Antibody, clone 4G10, agarose conjugate

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