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描述 产品目录编号 14-752 品牌系列 Upstate
商名 - Upstate
描述 GRK7 Protein, active, 10 µg 概述 N-terminal 6His-tagged, recombinant, full length human GRK7 expressed by baculovirus in Sf21 cells. 产品信息 演示 10μg of recombinant protein in 100 μl of 50 mM Tris-HCl pH 8.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. 应用 应用 Active, N-terminal 6His-tagged, recombinant, full length human GRK7 expressed by baculovirus in Sf21 cells. For use in Kinase Assays. 主要应用 - Kinase Assay
生物信息 来源 Recombinant, full length human GRK7 expressed by baculovirus in Sf21 cells. 品种 Human 特异活性 381U/mg, where one unit of GRK7 activity is defined as 1nmol phosphate incorporated into 2mg/ml casein per minute at 30°C with a final ATP concentration of 100μM. Entrez基因编号 - NM_139209.2
Entrez基因汇总 This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. It is specifically expressed in the retina and the encoded protein has been shown to phosphorylate cone opsins and initiate their deactivation. 基因符号 - GPRK7
- GRK7
蛋白靶 GRK7 纯化方法 Purified using Ni2+/NTA agarose. Purity 99% by SDS-PAGE and Coomassie blue staining. 靶子系列 AGC UniProt编号 - Q8WTQ7
UniProt汇总 FUNCTION: SwissProt: Q8WTQ7 # Phosphorylates cone opsins thereby initiating their deactivation.
SIZE: 553 amino acids; 62212 Da
SUBCELLULAR LOCATION: Membrane; Lipid-anchor.
TISSUE SPECIFICITY: Retina. Cones and rod.
SIMILARITY: SwissProt: Q8WTQ7 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 protein kinase domain. & Contains 1 RGS domain.产品使用声明 质量保证 Routinely evaluated by phosphorylation of 2 mg/mL casein.
Purity was assessed by SDS-PAGE and Coomassie blue staining using 3μg of GRK7.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 6 months at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap. 包装信息 数量 10 µg
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