merck millipore,默克密理博,14-744M,Haspin Protein, active, 250 µg

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Merck Millipore 默克密理愽

描述
产品目录编号	14-744M
品牌系列	Upstate
商名	Upstate
描述	Haspin Protein, active, 250 µg
概述	N-terminal 6His-tagged, recombinant, human Haspin, amino acids 471-end, expressed by baculovirus in Sf21 insect cells.

产品信息
演示	10 μg of enzyme in 6.3 μL of 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij 35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.

应用
应用	Active, N-terminal 6His-tagged, recombinant, human Haspin, amino acids 471-end, expressed by baculovirus in Sf21 insect cells. For use in Kinase Assays.
主要应用	Kinase Assay

生物信息
来源	Recombinant enzyme expressed in Sf21 cells
品种	Human
特异活性	2237U/mg, where one unit of Haspin activity is defined as 1 nmol phosphate incorporated into 500 μM RARTLSFAEPG per minute at 30°C with a final ATP concentration of 100 μM.
Entrez基因编号	NM_031965.2
基因符号	haspin GSG2 H-Haspin
蛋白靶	haspin
纯化方法	Purified using Ni²⁺/NTA agarose. Purity 83% by SDS-PAGE and Coomassie blue staining.
靶子系列	Misc
UniProt编号	Q8TF76
UniProt汇总	FUNCTION: SwissProt: Q8TF76 # Required for normal alignment of chromosomes at metaphase. Phosphorylates histone H3 'Thr-3' during mitosis. COFACTOR: Magnesium. SIZE: 798 amino acids; 88460 Da SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Strongly expressed in testis. Also present in thymus and bone marrow and low levels observed in prostate, intestine, lung, spleen and lymph node. Expressed in fetal skin, liver, kidney and small intestine and also in proliferating but not non-proliferating cell lines. PTM: Autophosphorylated on both serine and threonine residues (By similarity). Phosphorylated during mitosis. Phosphorylated upon DNA damage, probably by ATM or ATR. SIMILARITY: SwissProt: Q8TF76 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Haspin subfamily. & Contains 1 protein kinase domain.

产品使用声明
质量保证	Routinely evaluated by phosphorylation of 500 μM RARTLSFAEPG. Purity was assessed by SDS-PAGE and Coomassie blue staining using 3μg of Haspin.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 6 months at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap. Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.

包装信息
数量	250 µg
包装	Also available in 10 μg size (cat#14-744) and in bulk (cat# 14-744-K).

merck millipore,默克密理博,14-744M,Haspin Protein, active, 250 µg

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