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描述 产品目录编号 14-743 品牌系列 Upstate
商名 - Upstate
描述 GCK Protein, active, 10 µg 概述 N-terminal GST- tagged, recombinant, human GCK amino acids 1-473, expressed by baculovirus in Sf21 cells. Alternate Names - germinal center kinase
应用 应用 Active, N-terminal GST-tagged, recombinant, human GCK amino acids 1-473, expressed by baculovirus in Sf21 cells. For use in Kinase Assays. 主要应用 - Kinase Assay
生物信息 来源 Recombinant protein expressed in Sf21 cells 品种 Human 特异活性 2337 U/mg, where one unit of GCK activity is defined as 1nmol phosphate incorporated into 0.8 mg/mL myelin basic protein per minute at 30°C with a final ATP concentration of 100 μM. Entrez基因编号 - NM_004579.2
Entrez基因汇总 The protein encoded by this gene is a member of the serine/threonine protein kinase family. Although this kinase is found in many tissues, its expression in lymphoid follicles is restricted to the cells of germinal centre, where it may participate in B-cell differentiation. This kinase can be activated by TNF-alpha, and has been shown to specifically activate MAP kinases. This kinase is also found to interact with TNF receptor-associated factor 2 (TRAF2), which is involved in the activation of MAP3K1/MEKK1. 基因符号 - BL44
- MAP4K2
- GCK
- RAB8IP
蛋白靶 GCK 纯化方法 Purified using glutathione-agarose. Purity 52% by SDS-PAGE and Coomassie blue staining. 靶子系列 STE UniProt编号 - Q12851
UniProt汇总 FUNCTION: SwissProt: Q12851 # Enhances MAP3K1 oligomerization, which may relieve amino-terminal mediated MAP3K1 autoinhibition and lead to activation following autophosphorylation. May play a role in the regulation of vesicle targeting or fusion.
COFACTOR: Magnesium.
SIZE: 819 amino acids; 91586 Da
SUBUNIT: Interacts with TRAF2, MAP3K1 and RAB8A.
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Basolateral cell membrane; Peripheral membrane protein (By similarity). Golgi apparatus membrane; Peripheral membrane protein (By similarity).
TISSUE SPECIFICITY: Highly expressed in germinal center but not mantle zone B-cells. Also expressed in lung, brain and placenta and at lower levels in other tissues examined.
SIMILARITY: SwissProt: Q12851 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. & Contains 1 CNH domain. & Contains 1 protein kinase domain.产品使用声明 质量保证 Routinely evaluated by phosphorylation of 0.8 mg/mL myelin basic protein.
Purity was assessed by SDS-PAGE and Coomassie blue staining using 3 μg of active GCK.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 6 months at -70 °C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 °C.包装信息 数量 10 µg
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