merck millipore,默克密理愽
  • merck millipore,默克密理博,14-452,PDK1 Protein, active, 10 µg

  • merck millipore,默克密理博,14-452,PDK1 Protein, active, 10 µg

    产品名称：PDK1 Protein, active, 10 µg
    产品型号：14-452
    Active, N-terminal, His6-tagged, recombinant human PDK1, amino acids 52-end. For use in Kinase Assays.

    merck millipore,默克密理博,14-452,PDK1 Protein, active, 10 µg

  • 产品介绍
  • merck millipore,默克密理博,14-452,PDK1 Protein, active, 10 µg

    描述
    产品目录编号 14-452
    品牌系列 Upstate
    商名
    • Upstate
    描述 PDK1 Protein, active, 10 µg
    概述 N-terminal, His6-tagged, recombinant human PDK1, amino acids 52-end.
    产品信息
    演示 50mM Tris-HCl, pH 7.5, 0.15M NaCl, 0.1mM EGTA, 0.03% Brij-35, 0.1% 2-mercaptoethanol, 1mM benzamidine, 200μM PMSF and 5% glycerol
    应用
    应用 Active, N-terminal, His6-tagged, recombinant human PDK1, amino acids 52-end. For use in Kinase Assays.
    主要应用
    • Kinase Assay
    生物信息
    来源 Human PDK1 expressed by baculovirus in Sf21 insect cells
    品种 Human
    特异活性 For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
    Entrez基因编号
    • NM_031268.4
    • NM_002613.3
    基因符号
    • hPDK1
    • PDPK1
    • MGC20087
    • PDK1
    • PkB-like
    • PRO0461
    • MGC35290
    蛋白靶 PDK1
    纯化方法 Ni2+/NTA-agarose
    靶子系列 AGC
    UniProt编号
    • O15530
    UniProt汇总 FUNCTION: SwissProt: O15530 # Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.
    SIZE: 556 amino acids; 63152 Da
    SUBCELLULAR LOCATION: Cytoplasm. Membrane; Peripheral membrane protein. Note=Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.
    TISSUE SPECIFICITY: Appears to be expressed ubiquitously.
    PTM: Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.
    SIMILARITY: SwissProt: O15530 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily. & Contains 1 PH domain. & Contains 1 protein kinase domain.
    产品使用声明
    质量保证 Routinely evaluated by phosphorylation of PDKtide
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 6 months at -70°C
    包装信息
    数量 10 µg
    包装 Also available in 250 µg size --call for pricing and availability and reference catalog number 14-452M when ordering the 250 µg size.

    merck millipore,默克密理博,14-452,PDK1 Protein, active, 10 µg

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