• merck millipore,默克密理博,MABE341,Anti-Puromycin Antibody, clone 17H1
  • merck millipore,默克密理博,MABE341,Anti-Puromycin Antibody, clone 17H1

    产品名称:Anti-Puromycin Antibody, clone 17H1
    产品型号:MABE341
    Anti-Puromycin antibody, clone 17H1 detects puromycin incorporated into protein. Monoclonal antibodies to puromycin may also be used with standard immunochemical methods.

    merck millipore,默克密理博,MABE341,Anti-Puromycin Antibody, clone 17H1

  • 产品介绍
  • merck millipore,默克密理博,MABE341,Anti-Puromycin Antibody, clone 17H1

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    A, HWBRPurifiedMonoclonal Antibody
    描述
    产品目录编号 MABE341
    描述 Anti-Puromycin Antibody, clone 17H1
    背景信息 Puromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, that functions as a protein synthesis inhibitor that blocks translation through premature chain termination in the ribosome. Monoclonal antibodies to puromycin may be used with standard immunochemical methods to directly monitor translation, a method known as surface sensing of translation (SUnSET). Part of the molecule resembles the 3' end of the aminoacylated tRNA, making it useful for protein translation analysis. Puromycin induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells.
    产品信息
    格式 Purified
    控制
    • HEK293 cell lysates treated with Puromycin and Cyclohexamide, or with Puromycin only.
    演示 Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
    应用
    应用 Anti-Puromycin antibody, clone 17H1 detects puromycin incorporated into protein. Monoclonal antibodies to puromycin may also be used with standard immunochemical methods.
    主要应用
    • Western Blotting
    生物信息
    免疫原品种 Puromycin from Streptomyces alboniger
    克隆 17H1
    宿主 Rat
    同种型 IgG2aκ
    品种反应性 AllHuman
    Species Reactivity Note Demonstrated to react with Human test sample, preincubated with Puromycin. Predicted to react with all species when test sample is incubated with Puromycin.
    抗体类型 Monoclonal Antibody
    纯化方法 Protein G purified
    产品使用声明
    质量保证 Evaluated by Western Blotting in HEK293 cell lysates treated with Puromycin and Cyclohexamide, or with Puromycin only.

    Western Blotting Analysis: A 1:100,000 dilution of this antibody detected Puromycin-incorporated neosynthesized proteins in HEK293 cell lysates treated with Puromycin only. This antibody also detected small mounts of Puromycin-incorporated neosynthesized proteins in HEK293 cells treated with Puromycin and Cyclohexamide.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at 2-8°C from date of receipt.
    包装信息
    数量 100 µL

    merck millipore,默克密理博,MABE341,Anti-Puromycin Antibody, clone 17H1

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