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merck millipore,默克密理博,FCIM025168,FlowCellect™ Mouse Viable Treg Characterization Kit
产品名称：FlowCellect™ Mouse Viable Treg Characterization Kit
产品型号：FCIM025168
This FlowCellect Mouse Viable Treg Characterization Kit for Flow Cytometry includes three directly conjugated antibodies, Anti-CD4-PerCP/Cy5.5, Anti-CD25-PE & Anti-Foxp3-Alexa Fluor 488.
merck millipore,默克密理博,FCIM025168,FlowCellect™ Mouse Viable Treg Characterization Kit
- 产品介绍
描述 产品目录编号 FCIM025168 商名 - FlowCellect
描述 FlowCellect™ Mouse Viable Treg Characterization Kit 概述 Millipore’s FlowCellect™ Mouse Viable Treg Characterization kit includes three directly conjugated antibodies, Anti-CD4-PerCP/Cy5.5, Anti-CD25-PE, and Anti-Foxp3-Alexa Fluor® 488. A viability dye is also included to allow for the discrimination of only viable cells during sample analysis. This four color kit is designed to characterize viable Tregs within mixed cell populations.
Eliminating false results due to non-specific binding of antibodies to dead cells allows researchers to more accurately identify regulatory T-cells from different sample collections regardless of changes in viability between samples. By doing so, Tregs can now be analyzed across multiple samples with a normalized and accurate measurement of their expression levels of each of the antibodies in the kit.
The antibodies provided have been carefully titrated to ensure the ability to accurately measure the expression of all markers simultaneously. Sufficient reagents are provided to perform 25 four-color tests. Detailed assay instructions are included to assist in analysis and to ensure the correct cell concentration is obtained during acquisition of sample data.Alternate Names - Flow Cellect
背景信息 Regulatory T cells (Tregs) are a subpopulation of CD4+ T cells which constitutively express CD25 (IL-2 receptor alpha chain) and the transcription factor Foxp3. Characterization of these cells can be accomplished by performing multi-parameter flow cytometry to identify key combinations of the aforementioned surface and intracellular markers.
Tregs play a vital role in regulating the responses of the immune system. They suppress the activation of the immune system following infections, which helps maintain immune homeostasis. It has also been shown that some cancer patients have increased numbers of Tregs which may allow some malignant cells to escape the activation of the immune system due to the suppressive nature of the Tregs. Researchers are interested in the mechanism by which cancers are evading anti-tumor immune responses as these could aid in the design of alternate treatment methods.
A second and equally important function of Treg is promoting tolerance to self-antigens. Tregs act to ensure that self-antigens presented to the immune system do not illicit an immune response. This is yet another way that Tregs help to maintain immune homeostasis by actively suppressing the activation and expansion of self-reactive T cells.产品信息 部件 - 9. 1000X Fixable Viability Dye eFluor®660: (Part No.CF200098) One vial containing 50 µL of dye.
- 1. 20X Anti-CD4-PerCP/Cy5.5: (Part No. CS206550) 1 vial containing 150 µL.
- 2. 20X Anti-CD25-PE: (Part No. CS206540) One vial containing 150 µL antibody.
- 3. 20X Anti-Foxp3-Alexa Fluor® 488: (Part No. CS206539) One vial containing 150 µL antibody.
- 4. 10X Wash Buffer: (Part No. CS202123) One bottle containing 13 mL.
- 5. 4X Fixation/Permeabilization Buffer: (Part No. CS206472) One bottle containing 3.2 mL buffer.
- 6. Fixation/Permeabilization Diluent: (Part No. CS206473) One bottle containing 9.5 mL buffer.
- 7. 5X Assay Buffer: (Part No. CS202124) One 55 mL bottle
- 8. 10X Permeabilization Buffer: (Part No. CS206474) One bottle containing 6.5 mL buffer
检测方法 Fluorescent 应用 应用 This FlowCellect Mouse Viable Treg Characterization Kit for Flow Cytometry includes three directly conjugated antibodies, Anti-CD4-PerCP/Cy5.5, Anti-CD25-PE & Anti-Foxp3-Alexa Fluor 488. 主要应用 - Flow Cytometry
生物信息 宿主 Mixed Species 品种反应性 Human 抗体类型 Monoclonal Antibody 产品使用声明 质量保证 Lot to lot in-process testing on all kit components 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 4-8°C for antibodies and buffers 包装信息 数量 25 Tests / Kit
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