merck millipore,默克密理愽
  • merck millipore,默克密理博,14-450M,Cdk1/cyclin B Protein, active, 250 µg

  • merck millipore,默克密理博,14-450M,Cdk1/cyclin B Protein, active, 250 µg

    产品名称：Cdk1/cyclin B Protein, active, 250 µg
    产品型号：14-450M
    Active, C-terminal His6-tagged human full length Cdk1 & N-terminal GST-tagged human full length Cyclin B, for use in Kinase Assays.

    merck millipore,默克密理博,14-450M,Cdk1/cyclin B Protein, active, 250 µg

  • 产品介绍
  • merck millipore,默克密理博,14-450M,Cdk1/cyclin B Protein, active, 250 µg

    描述
    产品目录编号 14-450M
    品牌系列 Upstate
    商名
    • Upstate
    描述 Cdk1/cyclin B Protein, active, 250 µg
    概述 C-terminal His6-tagged human full length cdk1, and N-terminal GST-tagged human full length cyclin B
    应用
    应用 Active, C-terminal His6-tagged human full length Cdk1 & N-terminal GST-tagged human full length Cyclin B, for use in Kinase Assays.
    主要应用
    • Kinase Assay
    生物信息
    来源 expressed individually by baculovirus in Sf21 insect cells
    品种 Human
    特异活性 For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
    Entrez基因编号
    • NM_033379.2
    • NM_001786.2
    Entrez基因汇总 The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control.
    基因符号
    • CDC28A
    • CDC2
    • CDK1
    • MGC111195
    • DKFZp686L20222
    蛋白靶 CDK1/cyclinB
    纯化方法 Purified using Ni2+/NTA-agarose and GST-agarose, respectively. The cdk1 is then activated using CAK and repurified by Q Sepharose™ and Ni2+/NTA-agarose
    靶子系列 CMGC
    UniProt编号
    • P06493
    UniProt汇总 FUNCTION: SwissProt: P06493 # Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.
    SIZE: 297 amino acids; 34095 Da
    SUBUNIT: Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin. Interacts with DLG7. Isoform 2 is unable to complex with cyclin B1 and also fails to bind to the CDK inhibitor p21. Interacts with catalytically active CCNB1 and RALBP1 during mitosis to form an endocytotic complex during interphase.
    SUBCELLULAR LOCATION: Nucleus (By similarity).
    SIMILARITY: SwissProt: P06493 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. & Contains 1 protein kinase domain.
    产品使用声明
    质量保证 routinely evaluated by phosphorylation of the Histone H1 substrate
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 6 months at -70°C
    包装信息
    数量 250 µg
    包装 Also available in 10 μg size (cat#14-450) and in bulk (cat# 14-450-K).

    merck millipore,默克密理博,14-450M,Cdk1/cyclin B Protein, active, 250 µg

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