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merck millipore,默克密理博,HTS039RTA,Ready-to-Assay™ D2L Dopamine Receptor Frozen Cells
产品名称：Ready-to-Assay™ D2L Dopamine Receptor Frozen Cells
产品型号：HTS039RTA
Ready-to-Assay Human D2L GPCR frozen cells for Calcium Flux FLIPR Assays.
merck millipore,默克密理博,HTS039RTA,Ready-to-Assay™ D2L Dopamine Receptor Frozen Cells
- 产品介绍
重要规格表
应用说明 品种 宿主细胞 蛋白靶 Calcium Flux Assays Human Chem-7 D2L 描述 产品目录编号 HTS039RTA 商名 - Ready-to-Assay
描述 Ready-to-Assay™ D2L Dopamine Receptor Frozen Cells 背景信息 Millipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.
Dopamine is a catecholamine neurotransmitter that functions in the CNS to control locomotor, cognitive, emotional and neurendocrine processes, and in the periphery to modulate cardiovascular, renal and gastrointestinal processes. The biological activities of dopamine are mediated by a family of five GPCRs. The D1 and D5 subtypes couple to Gs to increase intracellular cAMP, whereas the D2, D3 and D4 subtypes couple to Gi to reduce cAMP (Missale et al., 1998). The D2 dopamine receptors have been of particular clinical interest due to their regulation of prolactin secretion and their affinity for antipsychotic drugs. The D2 receptor exists as two alternatively spliced isoforms differing in the insertion of a stretch of 29 amino acids in the third intracellular loop (D2S and D2L) (Giros et al., 1989; Grandy et al., 1989). which supports high levels of recombinant D2L expression and contains optimized levels of a recombinant promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at D2L.产品信息 部件 - Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
检测方法 Fluorescent 应用 应用 Ready-to-Assay Human D2L GPCR frozen cells for Calcium Flux FLIPR Assays. 应用说明 Calcium Flux Assays 生物信息 品种 Human Entrez基因编号 - NM_000795
GPCR类别 A 宿主细胞 Chem-7 蛋白靶 D2L 靶子系列 Dopamine 细胞系类型 - Ready-to-Assay
产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
- This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.
包装信息 数量 1 kit
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