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品种反应性 主要应用 宿主 格式 抗体类型 Eq, H, M, B, Ca WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 09-489 描述 Anti-phospho-AS160 (Ser666) Antibody Alternate Names - TBC1 domain family, member 4
- Acrg embryonic lethality minimal region ortholog
- Akt substrate of 160 kDa
- TBC (Tre-2, BUB2, CDC16) domain-containing protein
背景信息 AS160 (Akt Substrate of 160 kDa, TBC1D4) is a Rab-GAP protein that regulates the trafficking of GLUT4 (glucose transporter 4) to the plasma membrane. AS160 is known to be phosphorylated on at least 8 residues following stimulation from IGF-1, EGF, PMA, and AICAR (Chen, S., 2008). Thr642 is phosphorylated by Akt in response to IGF-1, while Ser341 and Thr642 of AS160 are phosphorylated by RSK (p90 ribosomal S6 kinase) in response to PMA. Ser588 has been shown to be phosphorylated by AMPK in vitro, though other kinases might be responsible as well. The phosphorylation site, Ser666, does not contain the Akt consensus sequence. Its phosphorylation is suppressed by AICAR and by combined mutations of TOS (mTOR signaling)-like sequence (FEMDI) and rapamycin (Geraghty, K.M., 2007). Additionally, its phosphorylation is markedly increased in response to IGF-1 and this phosphorylation is blocked in response to the PI3 Kinase inhibitor LY294002 (Geraghty, K.M., 2007). 产品信息 格式 Affinity Purified 演示 Affinity purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 This Anti-phospho-AS160 (Ser666) Antibody is validated for use in WB for the detection of phospho-AS160 (Ser666). 主要应用 - Western Blotting
生物信息 免疫原品种 Synthetic peptide corresponding to amino acids including and surrounding phosphorylated Ser666 of human AS160. 表位 Phosphorylated Ser666 宿主 Rabbit 特异性 This antibody detected AS160 when phosphorylated on Ser666. 品种反应性 HorseHumanMouseBovineCanine Species Reactivity Note Human. Other species have not been tested, but immunogen shares 79% homology with mouse and 90% homology with cow, horse, and dog. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_005535.1
基因符号 - OTTHUMP00000040818
- AS160
- DKFZp779C0666
- KIAA0603
- OTTHUMP00000018505
修改 - Phosphorylation
纯化方法 Affinity Purfied UniProt编号 - O60343
UniProt汇总 FUNCTION:May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake.
SUBCELLULAR LOCATION: Cytoplasm. Note= Isoform 2 shows a cytoplasmic perinuclear localization in a myoblastic cell line in resting and insulin-stimulated cells.
TISSUE SPECIFICITY: Widely expressed, but differential expression for isoforms 1 and 2, with highest overall expression of isoform 2 in most tissues. Isoform 1 is highly expressed in skeletal muscle and heart, but was not detectable in the liver nor in adipose tissue. Isoform 2 strongly expressed in adrenal and thyroid gland, and also in lung, kidney, colon, brain and adipose tissue. Moderate isoform 2 expression in skeletal muscle. Expressed in pancreatic Langerhans islets, including beta cells (at protein level). Expression is decreased by twofold in pancreatic islets in type 2 diabetes patients compared to control subjects.
PTM: Phosphorylated by AKT1; insulin-induced. Ref.8 Ref.10 Ref.15 Ref.16 Ref.17 Ref.18 Ref.19 Ref.20 Ref.21 Ref.22
Insulin-stimulated phosphorylation is required for SLC2A4/GLUT4 translocation.
Physiological hyperinsulinemia increases phosphorylation in skeletal muscle. Insulin-stimulated phosphorylation is reduced by 39% in type 2 diabetic patients.
Involvement in disease May be involved in atopic dermatitis (AD). Ref.13
SEQUENCE SIMILARITY: Contains 2 PID domains.
Contains 1 Rab-GAP TBC domain
产品使用声明 质量保证 Evaluated by western blot on untreated and IGF1 treated HEK293 cell lysate.
Western Blot Analysis:
1:250 dilution of this antibody was used to detect AS160 phosphorylated on Ser666 in IGF1-treated HEK293 cell lysate.
使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8ºC from date of receipt.
包装信息 数量 250 µL
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