merck millipore,默克密理博,07-850,Anti-phospho-LIMK 1/2 (Tyr507/Thr508) Antibody

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merck millipore,默克密理博,07-850,Anti-phospho-LIMK 1/2 (Tyr507/Thr508) Antibody
产品名称：Anti-phospho-LIMK 1/2 (Tyr507/Thr508) Antibody
产品型号：07-850
Detect phospho-LIMK 1/2 (Tyr507/Thr508) using this anti-phospho-LIMK 1/2 (Tyr507/Thr508) Antibody validated for use in WB.

merck millipore,默克密理博,07-850,Anti-phospho-LIMK 1/2 (Tyr507/Thr508) Antibody

产品介绍

merck millipore,默克密理博,07-850,Anti-phospho-LIMK 1/2 (Tyr507/Thr508) Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
H	WB	Rb	Affinity Purified	Polyclonal Antibody

描述
产品目录编号	07-850
品牌系列	Upstate
商名	Upstate
描述	Anti-phospho-LIMK 1/2 (Tyr507/Thr508) Antibody

产品信息
格式	Affinity Purified
演示	100 μL of affinity purified rabbit polyclonal IgG in 50% storage buffer (PBS (without Mg²⁺ and Ca²⁺), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide) and 50% glycerol.

应用
应用	Detect phospho-LIMK 1/2 (Tyr507/Thr508) using this anti-phospho-LIMK 1/2 (Tyr507/Thr508) Antibody validated for use in WB.
主要应用	Western Blotting
应用说明	Immunoblot Analysis: A 1:1000 dilution of this lot detected phosphorylated LIMK 1/2 (Tyr507/Thr508) in RIPA lysates of SDF-1α treated Jurkat cells.

生物信息
免疫原品种	Peptide corresponding to amino acid region encompassing the human, mouse, rat and chicken phospho-LIMK1/2 (Tyr507/Thr508)
宿主	Rabbit
特异性	Recognizes phosphorylated LIMK1/2 (Tyr507/Thr508)
同种型	IgG
品种反应性	Human
Species Reactivity Note	Predicted to cross-react with Mouse, Rat, and Frog (100% homologous) based on sequence homology.
抗体类型	Polyclonal Antibody
Entrez基因编号	NM_002314.2
Entrez基因汇总	There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene.
基因符号	LIMK-1 LIMK
修改	Phosphorylation
纯化方法	Immunoaffinity purified
UniProt编号	P53671 P53667
UniProt汇总	FUNCTION: SwissProt: P53671 # Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro. SIZE: 638 amino acids; 72232 Da SUBUNIT: Binds ROCK1 and LKAP. SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Isoform LIMK2a is distributed in the cytoplasm and the nucleus, and isoform LIMK2b occurs mainly in the cytoplasm and is scarcely translocated to the nucleus. TISSUE SPECIFICITY: Highest expression in the placenta; moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, colon, stomach, and spleen, while in brain, kidney, and placenta LIMK2b is the dominant form. In adult lung, both LIMK2a and LIMK2b is nearly equally observed. PTM: Phosphorylated on serine and/or threonine residues by ROCK1. SIMILARITY: SwissProt: P53671 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. & Contains 2 LIM zinc-binding domains. & Contains 1 PDZ (DHR) domain. & Contains 1 protein kinase domain.

产品使用声明
质量保证	Routinely evaluated by immunoblot.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

包装信息
数量	100 µL

merck millipore,默克密理博,07-850,Anti-phospho-LIMK 1/2 (Tyr507/Thr508) Antibody

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