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品种反应性 主要应用 宿主 格式 抗体类型 H IHC M Purified Monoclonal Antibody 描述 产品目录编号 MAB1254 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Chymase Antibody, clone B7 Alternate Names - Mast Cell Protease I
背景信息 Mast cell chymase (EC 3.4.21.39), a member of the granzyme subfamily of the S1 family of peptidases, cleaves C-terminal to Phenylalanine, Tyrosine, Tryptophan, and Leucine residues (in order of preference). Chymase is stored in Mast cell granules within the cell and is the major secreated Mast cell protease. Secreated Mast cell chymase is believed to be involved in extracellular matrix degradation, vasoactive peptide generation, and regulation of gland secreation. 产品信息 格式 Purified 控制 - POSITIVE CONTROL: lung, heart, skin, placenta.
演示 Purified by protein G affinity chromatography. Liquid in PBS with 20% glycerol, pH 7.4. 应用 应用 Anti-Chymase Antibody, clone B7 is an antibody against Chymase for use in DB, IH. 主要应用 - Immunohistochemistry
不建议的应用 - Western Blotting
应用说明 Dot blotting: 0.5-1.0 μg/mL.
Immunohistochemistry: 0.5-1.0 μg/mL. For optimal results, tissues or cytospin preparations should be fixed in Carnoy's Fluid (60% ethanol, 30% chloroform, 10% glacial acetic acid). Not recommended for use on formaldehyde fixed tissue.
Not recommended for use on Western blots.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Chymase purified from human skin. 克隆 B7 浓缩 2.4 mg/mL 宿主 Mouse 特异性 Reacts with human mast cell chymase. 同种型 IgG1κ 品种反应性 Human 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_001836.2
Entrez基因汇总 This gene product is a chymotryptic serine proteinase that belongs to the peptidase family S1. It is expressed in mast cells and thought to function in the degradation of the extracellular matrix, the regulation of submucosal gland secretion, and the generation of vasoactive peptides. In the heart and blood vessels, this protein, rather than angiotensin converting enzyme, is largely responsible for converting angiotensin I to the vasoactive peptide angiotensin II. Angiotensin II has been implicated in blood pressure control and in the pathogenesis of hypertension, cardiac hypertrophy, and heart failure. Thus, this gene product is a target for cardiovascular disease therapies. This gene maps to 14q11.2 in a cluster of genes encoding other proteases. 基因符号 - EC 3.4.21.39
- CMA1
- CYM
- MGC119890
- Alpha-chymase
- MCT1
- MGC119891
- CYH
UniProt编号 - P23946
UniProt汇总 FUNCTION: SwissProt: P23946 # Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion.
SIZE: 247 amino acids; 27325 Da
SUBCELLULAR LOCATION: Secreted. Cytoplasmic granule. Note=Mast cell granules.
TISSUE SPECIFICITY: Mast cells in lung, heart, skin and placenta.
SIMILARITY: SwissProt: P23946 ## Belongs to the peptidase S1 family. Granzyme subfamily. & Contains 1 peptidase S1 domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt. Antibody may also be stored at -20ºC in undiluted aliquots. 包装信息 数量 100 µg
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