merck millipore,默克密理博,07-741,Anti-AS160 (Rab-GAP) Antibody

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merck millipore,默克密理博,07-741,Anti-AS160 (Rab-GAP) Antibody
产品名称：Anti-AS160 (Rab-GAP) Antibody
产品型号：07-741
Detect AS160 (Rab-GAP) using this Anti-AS160 (Rab-GAP) Antibody validated for use in WB.

merck millipore,默克密理博,07-741,Anti-AS160 (Rab-GAP) Antibody

产品介绍

merck millipore,默克密理博,07-741,Anti-AS160 (Rab-GAP) Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
Ca, H, M, R	WB	Rb	Serum	Polyclonal Antibody

描述
产品目录编号	07-741
品牌系列	Upstate
商名	Upstate
描述	Anti-AS160 (Rab-GAP) Antibody
Alternate Names	TBC1 domain family, member Acrg embryonic lethality minimal region ortholog Akt substrate of 160 kDa TBC (Tre-2, BUB2, CDC16) domain-containing protein BUB2 CDC16) domain-containing protein TBC1 domain family
背景信息	AS160 (Akt Substrate of 160 kDa, Akt1S1), Rab GAP (GTPase activating protein), is phosphorylated on multiple sites by Akt in response to insulin in adipocytes and contraction in muscles. AS160 is the long sought after link between the upstream insulin signaling through insulin/PI3K/Akt and GLUT4 vessicle translocation to the cell membrane. It has 6 Akt phosphorylation consensus sequences that are believed to become phosphorylated upon insulin stimulation. This phosphorylation is required for translocation of GLUT4-containing vesicles to the cell membrane1. A mutation of two or more of these phosphorylation sites results in a dramatic decrease in AS160 activity and insulin-stimulated GLUT4 redistribution at the cell membrane2. AS160 has also shown to be important for GLUT4 expcytosis2.

产品信息
格式	Serum
控制	MDI-treated 3T3/L1 cell lysates.
演示	Rabbit polyclonal antiserum IgG in buffer containing 0.05% sodium azide and 30% glycerol.

应用
应用	Detect AS160 (Rab-GAP) using this Anti-AS160 (Rab-GAP) Antibody validated for use in WB.
主要应用	Western Blotting

生物信息
免疫原品种	KLH-conjugated, synthetic peptide corresponding to amino acids 1178-1189 (C-PTNDKAKAGNKP) of rat Akt substrate of 160kDa (AS160) (Rab-GAP) with a N-terminal cysteine for conjugation purposes.
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	Recognizes AS160 (Rab-GAP), Mr 160 kDa.
同种型	IgG
品种反应性	CanineHumanMouseRat
Species Reactivity Note	Human and mouse. Predicted to cross react with rat and dog based on sequence homology.
抗体类型	Polyclonal Antibody
Entrez基因编号	NP_055647
基因符号	OTTHUMP00000040818 AS160 DKFZp779C0666 KIAA0603 OTTHUMP00000018505
纯化方法	Unpurified
UniProt编号	O60343
UniProt汇总	FUNCTION: SwissProt: O60343 # May act as a GTPase-activating protein for Rab family protein(s). SIZE: 1298 amino acids; 146563 Da TISSUE SPECIFICITY: Expressed in heart, skeletal muscle and in immune tissues (spleen, lymph node and leukocytes). PTM: Phosphorylated by AKT1; insulin-induced. & Insulin-stimulated phosphorylation is required for GLUT4 translocation (By similarity). DISEASE: SwissProt: O60343 # May be involved in atopic dermatitis (AD). SIMILARITY: SwissProt: O60343 ## Contains 2 PID domains. & Contains 1 Rab-GAP TBC domain. MISCELLANEOUS: Subcellular redistribution from low density microsomes to the cytosol with insulin treatment (By similarity).

产品使用声明
质量保证	Routinely evaluated by Western Blot in RIPA lysates from 3T3/L1 cells differentiated into adipocytes. Western Blot Analysis: A 1:1000-1:5000 dilution of this lot detected AS160 (Rab-GAP) in RIPA lysates from 3T3/L1 cells differentiated into adipocytes by MDI treatment. This lot also detects AS160 (Rab-GAP) in HEK293 lysates.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.