- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M, H WB Rb Purified Polyclonal Antibody 描述 产品目录编号 07-278 Replaces 04-467 品牌系列 Upstate
商名 - Upstate
描述 Anti-phospho-LAT (Tyr191) Antibody 产品信息 格式 Purified 演示 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30% 应用 应用 This Anti-phospho-LAT (Tyr191) Antibody is validated for use in WB for the detection of phospho-LAT (Tyr191). 主要应用 - Western Blotting
生物信息 免疫原品种 peptide (SREpYVNVSPEQQ-C) corresponding to amino acids 188-195 of human LAT and amino acids 192-203 of mouse LAT 宿主 Rabbit 特异性 LAT phosphorylated on tyrosine 191 同种型 IgG 品种反应性 MouseHuman 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_014387
Entrez基因汇总 The protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement. Alternative splicing results in multiple transcript variants encoding different isoforms. 基因符号 - p36-38
- LAT
- LAT1
- pp36
修改 - Phosphorylation
纯化方法 Protein A chromatography UniProt编号 - O43561
UniProt汇总 FUNCTION: SwissProt: O43561 # Required for TCR (T-cell antigen receptor)- and pre-TCR- mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.
SIZE: 262 amino acids; 27930 Da
SUBUNIT: When phosphorylated, interacts directly with the PIK3R1 subunit of phosphoinositide 3-kinase and the SH2 domains of GRB2, GRAP, GRAP2, PLCG1 and PLCG2. Interacts indirectly with CBL, SOS, VAV, and LCP2. Interacts with SHB and SKAP2.
SUBCELLULAR LOCATION: Cell membrane; Single-pass type III membrane protein. Note=Present in lipid rafts.
TISSUE SPECIFICITY: Expressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. Present in T-cells but not B-cells (at protein level).
PTM: Phosphorylated on tyrosines by ZAP-70 upon TCR activation, or by SYK upon other immunoreceptor activation; which leads to the recruitment of multiple signaling molecules. Is one of the most prominently tyrosine-phosphorylated proteins detected following TCR engagement. & Palmitoylation of Cys-26 and Cys-29 is required for raft targeting and efficient phosphorylation.
MISCELLANEOUS: Engagement of killer inhibitory receptors (KIR) disrupts the interaction of PLCG1 with LAT and blocks target cell- induced activation of PLC, maybe by inducing the dephosphorylation of LAT.产品使用声明 质量保证 routinely evaluated by immunoblot on RIPA lysates from Jurkat cells stimulated with anti-T-cell receptor monoclonal antibody, clone C305 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 2 years at -20°C 包装信息 数量 200 µg
上一件merck millipore产品：merck millipore,默克密理博,07-360,Anti-acetyl-Histone H3 (Lys27) Antibody
下一件merck millipore产品：merck millipore,默克密理博,RM0XPSMAG-17K,MILLIPLEX MAP Rat/Mouse Oxidative Phosphorylation (OXPHOS) Magnetic Bead Panel - Cellular Metabolism Multiplex Assay
相关产品
merck millipore,默克密理博,401484,InSolution™ IKK-2 Inhibitor IV - Calbiochem
merck millipore,默克密理博,SF-124,RNA POL II; Mouse, Cyanine 5, RNA Detection Probe,SmartFlare
merck millipore,默克密理博,14-602,PI3 Kinase (p110α/p85α) Protein, active, 20 µg
merck millipore,默克密理博,70573,Clonables™ 2X Ligation Premix - Novagen
merck millipore,默克密理博,07-360,Anti-acetyl-Histone H3 (Lys27) Antibody
merck millipore,默克密理博,PC284,Anti-AML1 (Ab-1) Rabbit pAb
merck millipore,默克密理博,MAB3510P,Anti-BrdU Antibody, clone BU-1, Peroxidase conjugated
merck millipore,默克密理博,AB16529,Anti-MyD88 Antibody, internal sequence
merck millipore,默克密理博,MAB3061,Anti-Fas Antibody, clone SM1/1