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描述 概述 The Clonables™ 2X Ligation Premix is a single solution containing optimized concentrations of the highest quality T4 DNA ligase, buffer, stabilizer, and cofactors needed for efficient ligation of any type of compatible DNA ends. The premix is tested for ligation of compatible 2- to 4-base cohesive DNA ends as well as blunt ends and single-base overhangs found on some PCR products. Under most conditions excellent ligation is achieved in only 15 minutes; with T/A or U/A overhangs more colonies can be obtained by incubating up to two hours. Although maximal efficiencies are obtained using NovaBlue Singles Competent Cells, this reagent is compatible with transformation of any type of chemically competent cells provided by the user. The high performance, minimal pipetting requirement, and single-addition format make the premix suitable for high-throughput applications.
One vial of the 2X Ligation Premix (Cat. No. 70573-3) contains enough reagent for 11 ligation reactions (10-µl scale).产品目录编号 70573 品牌系列 Novagen®
储存和货运信息 货运代码 Shipped with Blue Ice or with Dry Ice
毒性 Irritant
储存 ≤ -70°C
避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
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