- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, M, Ca, Eq WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 07-2134 描述 Anti-phospho-PLCγ-1 Antibody (Tyr783) Alternate Names - triphosphoinositide phosphodiesterase
- 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1
- Phosphoinositide phospholipase C
- Phospholipase C-gamma-1
- monophosphatidylinositol phosphodiesterase
- phosphatidylinositol phospholipase C
- phosphoinositidase C
- phospholipase C gamma 1
- phospholipase C, gamma 1
- phospholipase C, gamma 1 (formerly subtype 148)
- phospholipase C-148
背景信息 Phospholipase C gamma 1 is a tyrosine kinase substrate for many receptor and nonreceptor tyrosine kinases. Activation of the enzyme produces two second messenger molecules, inositol 1,4,5-trisphosphate and diacylglycerol, which provoke the mobilization of intracellular Ca2+ and activation of protein kinase C. Although the mammalian genome encodes 10 known phosphoinositide specific PLCs, only the gamma 1 and gamma 2 isoforms are regulated by tyrosine kinase activity. PLC beta isoform activity is controlled by heterotrimeric G protein coupled receptors, while the mechanism of regulation of PLCgamma activity is unknown. 产品信息 格式 Affinity Purified 控制 - A431 cell lysate
演示 Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-glycine, pH 7.4, 150 mM NaCl and 0.05% sodium azide. 应用 应用 This phospho-PLCγ-1 antibody is validated for use in WB for the detection of the phospho-PLCγ-1 protein. 主要应用 - Western Blotting
应用说明 Western Blot SNAP ID: 1:1,000 dilution of this antibody from a previous lot detected phospho-PLCγ-1 (Tyr783) on of untreated and EGF treated A431 cell lysate.
Western Blot SNAP ID Analysis:生物信息 免疫原品种 KLH conjugated linear peptide corresponding to phospho-PLCγ-1 (Tyr783). 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes phospho-PLCγ-1 (Tyr783). 品种反应性 RatHumanMouseCanineHorse Species Reactivity Note Proven to react with human. Predicted to react with Mouse, Rat, Equine, and Canine based on sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_002651
Entrez基因汇总 The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene.
基因符号 - inositoltrisphosphohydrolase
- 1-phosphatidyl-D-myo-inositol-4,5-bisphosphate
- EC 3.1.4.11
- OTTHUMP00000031787
- OTTHUMP00000178982
- PLC-148
- PLC-II
- PLC-gamma-1
- PLC1
- PLC148
- PLCgamma1
修改 - Phosphorylation
纯化方法 Affinity Purfied UniProt编号 - P19174
UniProt汇总 FUNCTION: PLC-gamma is a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.
CATALYTIC ACTIVITY: 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.
COFACTOR: Calcium.
SUBUNIT STRUCTURE: Interacts with AGAP2 via its SH3 domain By similarity. Interacts with phosphorylated LAT upon TCR activation. Interacts with the Pro-rich domain of TNK1 via its SH3 domain. Associates with BLNK, VAV1, GRB2 and NCK1 in a B-cell antigen receptor-dependent fashion. Interacts with CBLB in activated T-cells; which inhibits phosphorylation. Interacts with SHB. Interacts via its SH3 domain with the Arg/Gly-rich-flanked Pro-rich domains of KHDRBS1/SAM68. This interaction is selectively regulated by arginine methylation of KHDRBS1/SAM68. Interacts with INPP5D/SHIP1 and CLNK By similarity. Interacts with RALGPS1. Interacts (via SH3 domain) with HEV ORF3 protein.
DOMAIN: The SH3 domain mediates interaction with CLNK By similarity. The SH3 domain also mediates interaction with RALGPS1.
PTM: The receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors.
Ubiquitinated by CBLB in activated T-cells By similarity.
SEQUENCE SIMILARITIES: Contains 1 C2 domain.
Contains 1 EF-hand domain.
Contains 2 PH domains.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain.
产品使用声明 质量保证 Evaluated by Western Blot in A431 cell lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected phospho-PLCγ-1 (Tyr783) on untreated and EGF treated A431 cell lysate.
使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain refrigerated at 2-8°C for up to 6 months. 包装信息 数量 100 µL
上一件merck millipore产品：merck millipore,默克密理博,ABC14,Anti-ATG5 Antibody
下一件merck millipore产品：merck millipore,默克密理博,SJHVM4710,Millicup-HV，0.45 µm，PVDF，非无菌
相关产品
merck millipore,默克密理博,17-229,Acetyl-Histone H4 Immunoprecipitation (ChIP) Assay Kit
merck millipore,默克密理博,07-550,Anti-SUV39H1 Antibody
merck millipore,默克密理博,AB9882,Anti-Huntingtin-Interacting Protein 1 Related (HIP1R) Antibody
merck millipore,默克密理博,ABC14,Anti-ATG5 Antibody
merck millipore,默克密理博,SJHVM4710,Millicup-HV，0.45 µm，PVDF，非无菌
merck millipore,默克密理博,MAB5598,Anti-HCN3 Antibody, clone TLL 6C5
merck millipore,默克密理博,AB15454,Anti-Proteolipid Protein Antibody
merck millipore,默克密理博,05-830,Anti-Neurofilament L Antibody, clone DA2
