- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
-
-
merck millipore,默克密理博,ABE252,Anti-Nanos homolog 2 Antibody
产品名称：Anti-Nanos homolog 2 Antibody
产品型号：ABE252
Anti-Nanos homolog 2 Antibody is a Rabbit Polyclonal Antibody for detection of Nanos homolog 2 also known as Nanos Homolog 2, Nos-2 & has been validated in WB & IHC.
merck millipore,默克密理博,ABE252,Anti-Nanos homolog 2 Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Po, H, M, B IHC, WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 ABE252 描述 Anti-Nanos homolog 2 Antibody Alternate Names - Nos-2
- Nanos Homolog 2
背景信息 Nanos homolog 2 (NANOS2) is a zinc-finger RNA-binding protein that is expressed only in male gonads. Nanos homolog 2 opposes RA/Stra8 signaling that promotes meiosis and development of female germ cells. Nanos homolog 2 inhibits meiosis by actively suppressing the expression of mRNAs required for meiosis. Previous studies have shown that Nanos homolog 2 interacts with components of the CCR4-NOT complex and is involved in deadenylating and destabilizing mRNA molecules involved in meiosis. Furthermore, Nanos homolog 2 may mediate degradation of these RNA molecules by P-bodies. Hence, Nanos homolog 2 plays an important role in male sex determination. 产品信息 格式 Affinity Purified 控制 - Human Testis Tissue Lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-Nanos homolog 2 Antibody is a Rabbit Polyclonal Antibody for detection of Nanos homolog 2 also known as Nanos Homolog 2, Nos-2 & has been validated in WB & IHC. 主要应用 - Immunohistochemistry
- Western Blotting
应用说明 Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected Nanos homolog 2 in rat seminiferous tubules. 生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to the N-terminus of human Nanos homolog 2. 表位 N-terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes Nanos homolog 2 at the N-terminus. 品种反应性 PigHumanMouseBovine Species Reactivity Note Demonstrated to react in Human and Mouse. Predicted to react with Bovine and Porcine based on 100% sequence homology. Other homologies: Chinese Hamster, Marmoset, Giant Panda, and Rabbit (83% sequence homology). 抗体类型 Polyclonal Antibody Entrez基因编号 - Np_001025032
基因符号 - Nos2
- Nanos2
纯化方法 Affinity purified UniProt编号 - P60321
UniProt汇总 FUNCTION: Plays a key role in the sexual differentiation of germ cells by promoting the male fate but suppressing the female fate. Represses the female fate pathways by suppressing meiosis, which in turn results in the promotion of the male fate. Maintains the suppression of meiosis by preventing STRA8 expression, which is required for premeiotic DNA replication, after CYP26B1 is decreased. Regulates the localization of the CCR4-NOT deadenylation complex to P-bodies and plays a role in recruiting the complex to trigger the degradation of mRNAs involved in meiosis. Required for the maintenance of the spermatogonial stem cell population. Not essential for the assembly of P-bodies but is required for the maintenance of their normal state (By similarity).
SUBUNIT STRUCTURE: Interacts with CNOT1, CNOT3, CNOT6L, CNOT7 and CNOT9 (By similarity).
SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm › P-body (By similarity). Cytoplasm › perinuclear region. Note: Localizes at P-bodies during gonocyte development (By similarity). More abundant in perinuclear region of the cytoplasm of the germ cells of the adult testis.
TISSUE SPECIFICITY: Testis and ovary. Expression found in several spermatogenic stages: in cells on the periphery of the tubules which could correspond to spermatogonia, in spermatocytes and in round spermatids (at protein level).
DEVELOPMENTAL STAGE: Fetal ovary and fetal testis. Present in all germ cells of seminiferous tubules of the 24-week fetus (at protein level).
DOMAIN: The Nanos-type zinc finger is composed of two C2HC motifs, each motif binding one molecule of zinc. It is essential for the translation repression activity of the protein (By similarity).
SEQUENCE SIMLARITIES: Belongs to the nanos family.
Contains 1 nanos-type zinc finger.产品使用声明 质量保证 Evaluated by Western Blot in human testis tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Nanos homolog 2 on 10 µg of human testis tissue lysate.储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,05-830,Anti-Neurofilament L Antibody, clone DA2
下一件merck millipore产品：merck millipore,默克密理博,14-415,Ral Assay Reagent (Ral BP1, agarose)
相关产品
merck millipore,默克密理博,07-2134,Anti-phospho-PLCγ-1 Antibody (Tyr783)
merck millipore,默克密理博,SJHVM4710,Millicup-HV，0.45 µm，PVDF，非无菌
merck millipore,默克密理博,MAB5598,Anti-HCN3 Antibody, clone TLL 6C5
merck millipore,默克密理博,AB15454,Anti-Proteolipid Protein Antibody
merck millipore,默克密理博,05-830,Anti-Neurofilament L Antibody, clone DA2
merck millipore,默克密理博,14-415,Ral Assay Reagent (Ral BP1, agarose)
merck millipore,默克密理博,HTS201M,ChemiSCREEN™ Membrane Preparation Recombinant Human GPR109A Receptor
merck millipore,默克密理博,05-919,Anti-T-Cell Receptor Antibody, clone C305
merck millipore,默克密理博,IF011,Interferon-β Protein, Recombinant mouse