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品种反应性 主要应用 宿主 格式 抗体类型 H ELISA, ICC Rb Purified Polyclonal Antibody 描述 产品目录编号 07-2066 描述 Anti-DOT1L Antibody Alternate Names - DOT1-like protein <br />DOT1-like, histone H3 methyltransferase<br />DOT1-like, histone H3 methyltransferase (S. cerevisiae)<br />Histone H3-K79 methyltransferase<br />Lysine N-methyltransferase 4<br />histone methyltransferase DOT1L
背景信息 DOT1L (DOT1-like, the mammalian homologue of yeast DOT1), is a DNA-binding protein that acts as a histone methyltransferase. DOT1L di- and trimethylates histone H3 on lysine 79, and is thought to be the sole enzyme responsible for this mark. DOT1L and H3K79 methylation play important roles in heterochromatin formation and in embryonic development. In contrast to other lysine histone methyltransferases, DOT1L does not contain a SET domain, suggesting a unique mechanism for methylation of lysines in histones DOT1L is associated with activating genes associated with acute leukemia by deregulation of cancer-relevant genes and binding with several MLL fusion partners. In yeast, Dot1-mediated H3K79 methylation is associated with telomere silencing, meiotic checkpoint control, and DNA damage response. 产品信息 格式 Purified 演示 Purified polyclonal antibody supplied in PBS with 0.09% sodium azide. 应用 应用 Use Anti-DOT1L Antibody (Rabbit Polyclonal Antibody) validated in ICC, ELISA to detect DOT1L also known as DOT1-like histone H3 methyltransferase. 主要应用 - ELISA
- Immunocytochemistry
应用说明 ELISA:
This antibody was reported by an outside laboratory to be suitable for ELISA AT A 1:1,000 dilution.生物信息 免疫原品种 The immunogen was a KLH-conjugated synthetic peptide corresponding to the C-terminal region of human DOT1L. 表位 C-terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes the C-terminal region of human DOT1L. 品种反应性 Human Species Reactivity Note Human. Not tested in other species. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_115871
基因符号 - KMT4
- DOT1L
- DOT1
纯化方法 Protein G chromatography UniProt编号 - Q8TEK3
UniProt汇总 FUNCTION: Histone methyltransferase. Methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones. Binds to DNA.
CATALYTIC ACTIVITY: S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N(6)-methyl-L-lysine. Ref.1
SUBCELLULAR LOCATION: NucleusProbable.
MISCELLANEOUS: In contrast to other lysine histone methyltransferase, it does not contain a SET domain, suggesting the existence of another mechanism for methylation of lysine residues of histones.
SEQUENCE SIMILARITIES: Belongs to the DOT1 family.
SEQUENCE CAUTION: The sequence AAC08316.1 differs from that shown. Reason: Erroneous gene model prediction.
产品使用声明 质量保证 Immunocytochemistry:
Gastric mucosal tissue incubated with anti-DOT1L stained strongly positive on nuclei.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 6 months at 2-8°C from date of receipt. For long term storage, store at -20ºC. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. 包装信息 数量 100 µg
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