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品种反应性 主要应用 宿主 格式 抗体类型 H, Av WB M Purified Monoclonal Antibody 描述 产品目录编号 05-377 品牌系列 Upstate
商名 - Upstate
描述 Anti-Rb Antibody, clone XZ-77 产品信息 格式 Purified 演示 0.1M Tris-glycine, pH 7.4, 0.15M NaCl with 0.05% sodium azide 应用 应用 Anti-Rb Antibody, clone XZ-77 is a high quality Mouse Monoclonal Antibody for the detection of Rb & has been validated in IP & WB. 主要应用 - Western Blotting
生物信息 免疫原品种 Purified recombinant retinoblastoma (Rb) protein corresponding to amino acid residues 387-928 expressed in E. coli. 克隆 clone XZ-77 宿主 Mouse 特异性 human cell retinoblastoma gene product in transformed human cell lines except for some types of Rb and breast cancer cells which contain inactivated or rearranged retinoblastoma gene; this antibody will immunoprecipitate and immunoblot the phosphorylated and hypophosphorylated forms of human Rb 同种型 IgG2aκ 品种反应性 HumanAvian 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_000321
Entrez基因汇总 Retinoblastoma (RB) is an embryonic malignant neoplasm of retinal origin. It almost always presents in early childhood and is often bilateral. Spontaneous regression ('cure') occurs in some cases. The retinoblastoma gene RB was the first tumor suppressor gene cloned, and is a negative regulator of the cell cycle through its ability to bind the transcription factor E2F (MIM 189971) and repress transcription of genes required for S phase (Hanahan and Weinberg, 2000 [PubMed 10647931]).[supplied by OMIM] 基因符号 - OSRC
- RB1
- RB
- PP110
- P105-RB
- retinoblastoma-1
纯化方法 Protein G chromatography UniProt编号 - P06400
UniProt汇总 FUNCTION: SwissProt: P06400 # Key regulator of entry into cell division that acts as a tumor suppressor. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Also acts as a transcription repressor of E2F target genes by recruiting chromatin-modifying enzymes to promoters. Inhibits the intrinsic kinase activity of TAF1. Forms a complex with adenovirus E1A and with SV40 large T antigen. May bind and modulate functionally certain cellular proteins with which T and E1A compete for pocket binding.
SIZE: 928 amino acids; 106159 Da
SUBUNIT: Interacts preferentially with transcription factor E2F1. The unphosphorylated form interacts with ARID3B, JARID1A, SUV39H1, MJD2A/JHDM3A and THOC1. Interacts with the N-terminal domain of TAF1. Interacts with AATF, DNMT1, LIN9, LMNA, SUV420H1, SUV420H2, PELP1 and TMPO-alpha. May interact with KNTC2. Interacts with EID1 and ZUBR1. Interacts with ARID4A and JARID1B.
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Expressed in the retina.
PTM: Phosphorylated from S to M phase of the cell cycle and is dephosphorylated in G1. T, but not E1A, binds only to the unphosphorylated form.
DISEASE: SwissProt: P06400 # Defects in RB1 are the cause of childhood cancer retinoblastoma (RB) [MIM:180200]. RB is a congenital malignant tumor that arises from the nuclear layers of the retina. It occurs in about 1:20'000 live births and represents about 2% of childhood malignancies. It is bilateral in about 30% of cases. Although most RB appear sporadically, about 20% are transmitted as an autosomal dominant trait with incomplete penetrance. The diagnosis is usually made before the age of 2 years when strabismus or a gray to yellow reflex from pupil (cat eye) is investigated. & Defects in RB1 are a cause of bladder cancer [MIM:109800]. & Defects in RB1 are a cause of osteogenic sarcoma [MIM:259500].
SIMILARITY: SwissProt: P06400 ## Belongs to the retinoblastoma protein (RB) family.产品使用声明 质量保证 routinely evaluated in immunoblot on HeLa nuclear extract 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 2 years at -20°C 包装信息 数量 200 µg
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