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品种反应性 主要应用 宿主 格式 抗体类型 H WB, ICC, ELISA Rb Purified Polyclonal Antibody 描述 产品目录编号 07-2046 描述 Anti-EHMT1 Antibody Alternate Names - euchromatic histone-lysine N-methyltransferase 1
- G9a like protein
- G9a-like protein 1
- H3-K9-HMTase 5
- Histone H3-K9 methyltransferase 5
- Lysine N-methyltransferase 1D
- euchromatic histone methyltransferase 1
背景信息 EHMT1 (euchromatic histone methyltransferase 1), also known as G9a-like protein (GLP1), is a histone methyltransferase. This protein specifically methylates Lysine 9 of histone H3 in vitro. H3Lys9 methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. EHMT1 is thought to be targeted to histone H3 by different DNA-binding proteins including E2F6, MGA, MAX and/or DP1. During G0 phase, it contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in the G0/G1 transition of the cell cycle. EMHT1/GLP1 is phosphorylated upon DNA damage, probably by ATM or ATR. Defects in EHMT1 result in severe mental retardation, hypotonia, and epileptic seizures. 产品信息 格式 Purified 控制 - A431, HeLa, or NIH/3T3 cells
演示 Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. 应用 应用 Use Anti-EHMT1 Antibody (Rabbit Polyclonal Antibody) validated in ICC, ELISA, WB to detect EHMT1 also known as H3-K9-HMTase 5, Histone H3-K9 methyltransferase 5. 主要应用 - Western Blotting
- Immunocytochemistry
- ELISA
应用说明 Western Blot Analysis
This antibody recognized EMHT1 in transfected 293 cell lysates.
ELISA:
This antibody is reported by an
outside laboratory to be suitable for ELISA
生物信息 免疫原品种 The immunogen was a KLH-conjugated synthetic peptide corresponding to an internal region of human EHMT1. 表位 Internal 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 品种反应性 Human Species Reactivity Note Recognizes human and murine EMHT1. Not tested in other species. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_079033
Entrez基因汇总 The protein encoded by this gene is a histone methyltransferase that is part of the E2F6 complex, which represses
transcription. The encoded protein methylates the Lys-9 position of histone H3, which tags it for transcriptional repression. This protein may be involved in the silencing of MYC- and E2F-responsive
genes and therefore could play a role in the G0/G1 cell cycle transition. Defects in this gene are a cause of chromosome 9q subtelomeric deletion syndrome (9q-syndrome). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
基因符号 - bA188C12.1
- DEL9q34
- DKFZp667M072
- EC 2.1.1.43
- EUHMTASE1
- Eu-HMTase1
- FLJ12879
- FP13812
- GLP
- GLP1
- KIAA1876
- KMT1D
- OTTHUMP00000022711
纯化方法 Protein A chromatography UniProt编号 - Q9H9B1
UniProt汇总 FUNCTION:Histone methyltransferase. Methylates 'Lys-9' of histone H3 (in vitro). H3 'Lys-9' methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in G0/G1 transition in cell cycle.
Catalytic activity S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N(6)-methyl-L-lysine. Ref.1
SUBUNIT STRUCTURE: Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2, MBLR, L3MBTL2 and YAF2. Interacts with WIZ and EHMT2. Ref.10
SUBCELLULAR LOCATION: Nucleus. Note= Associates with euchromatic regions.
TISSUE SPECIFICITY: Widely expressed. Ref.5
DOMAIN:The SET domain mediates interaction with WIZ.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.11
DISEASE: Defects in EHMT1 are the cause of chromosome 9q subtelomeric deletion syndrome (9q- syndrome) [MIM:610253]. Common features seen in these patients are severe mental retardation, hypotonia, brachy(micro)cephaly, epileptic seizures, flat face with hypertelorism, synophrys, anteverted nares, cupid bow or tented upper lip, everted lower lip, prognathism, macroglossia, conotruncal heart defects, and behavioral problems.
Sequence similarities Belongs to the histone-lysine methyltransferase family.
Contains 8 ANK repeats.
Contains 1 pre-SET domain.
Contains 1 SET domain.
SEQUENCE CAUTION:The sequence CAD28534.1 differs from that shown. Reason: Miscellaneous discrepancy. Intron retention.
产品使用声明 质量保证 Immunocytochemistry
Detects EHMT1 in the nuclei of A431, HeLa, or NIH/3T3 cells in immunofluorescent analysis.
使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial to pellet the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
包装信息 数量 100 µg
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