merck millipore,默克密理愽
  • merck millipore,默克密理博,07-1820,Anti-EEA1 Antibody

  • merck millipore,默克密理博,07-1820,Anti-EEA1 Antibody

    产品名称：Anti-EEA1 Antibody
    产品型号：07-1820
    This Anti-EEA1 Antibody is validated for use in WB, IC for the detection of EEA1.

    merck millipore,默克密理博,07-1820,Anti-EEA1 Antibody

  • 产品介绍
  • merck millipore,默克密理博,07-1820,Anti-EEA1 Antibody

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    M, HICC, WBRbAffinity PurifiedPolyclonal Antibody
    描述
    产品目录编号 07-1820
    描述 Anti-EEA1 Antibody
    Alternate Names
    • Zinc finger FYVE domain-containing protein 2
    • early endosome antigen 1
    • early endosome-associated protein
    • early endosome antigen 1, 162kD
    • Endosome-associated protein p162
    背景信息 Early endosome antigen 1 (EEA1) is a 170 kDa coiled-coil protein belonging to a group of early endosomal Rab5 effector proteins. EEA1 has a putative role in the tethering/docking of endocytic vesicles prior to their fusion with early endosomes. This protein is characterized as containing a zinc finger-like domain and coiled-coil regions. The zinc finger-like domain, also known as the FYVE finger, is a motif rich in cystine, is a necessary component of EEA1’s ability for endosomal targeting, and is specifically known for its dual interaction with Rab5 GTPase and phosphotidylinositol 3-phosphate (Ptdlns-3-P).
    产品信息
    格式 Affinity Purified
    控制
    • BALB 3T3/A31 cell lysate
    演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
    应用
    应用 This Anti-EEA1 Antibody is validated for use in WB, IC for the detection of EEA1.
    主要应用
    • Immunocytochemistry
    • Western Blotting
    应用说明 Western Blot (SNAP ID) Analysis: 1:250 dilution from a previous lot detected EEA1 on 10 µg of C2C12 cell lysate.

    Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected EEA1 in HeLa, A431, and C2C12 cells.
    生物信息
    免疫原品种 GST-tagged recombinant protein corresponding to the C-terminus of human EEA1.
    表位 C-terminus
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Rabbit
    特异性 This antibody recognizes EEA1.
    品种反应性 MouseHuman
    Species Reactivity Note Demonstrated to react with Human and Mouse.
    Equine and Canine (99% sequence homology). Rat (98% sequence homology). Bovine (97% sequence homology).
    抗体类型 Polyclonal Antibody
    Entrez基因编号
    • NP_003557
    基因符号
    • MSTP105
    • EEA1
    • ZFYVE2
    • MST105
    纯化方法 Affinity Purfied
    UniProt编号
    • Q15075
    UniProt汇总 FUNCTION: Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.

    SUBUNIT STRUCTURE: Homodimer. Binds STX6. Binds RAB5A, RAB5B, RAB5C and RAB22A that have been activated by GTP-binding.

    SUBCELLULAR LOCATION: Cytoplasm. Early endosome membrane; Peripheral membrane protein.

    DOMAIN: The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate.

    MISCELLANEOUS: Antibodies against EEA1 are found in sera from patients with subacute cutaneous lupus erythematosus and other autoimmune diseases.

    SEQUENCE SIMILARITIES: Contains 1 C2H2-type zinc finger.

    Contains 1 FYVE-type zinc finger.
    产品使用声明
    质量保证 Evaluated by Western Blot in BALB 3T3/A31 cell lysate.

    Western Blot Analysis: A 1:1000 dilution of this antibody detected EEA1 on 10 µg of BALB 3T3/A31 cell lysate.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at 2-8°C from date of receipt.
    包装信息
    数量 100 µL

    merck millipore,默克密理博,07-1820,Anti-EEA1 Antibody

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