merck millipore,默克密理博,03-116,RIPAb+™ STAU1 (Staufen 1)

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merck millipore,默克密理博,03-116,RIPAb+™ STAU1 (Staufen 1)
产品名称：RIPAb+™ STAU1 (Staufen 1)
产品型号：03-116

merck millipore,默克密理博,03-116,RIPAb+™ STAU1 (Staufen 1)

产品介绍

merck millipore,默克密理博,03-116,RIPAb+™ STAU1 (Staufen 1)

重要规格表

品种反应性	主要应用
H	WB

描述
产品目录编号	03-116
商名	Upstate RIPAb+
描述	RIPAb+™ STAU1 (Staufen 1)
概述	RIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes a negative control antibody to ensure specificity of the RIP reaction and is verified for the co-immunoprecipitation of RNA associated specifically with the immunoprecipitated RNA binding protein of interest. Where appropriate, the RIPAb+ set also includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully co-precipitating the specific RNA targets, such as messenger RNAs. The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. If a target specific assay is not provided, the RIPAb+ kit is validated using an automated microfluidics-based assay by enrichment of detectable RNA over control immunoprecipitation. STAU1 (Staufen 1) is a RNA-binding protein implicated in the transport and/or localization of mRNA via the microtubule network to the rough endoplasmic reticulum.

产品信息
格式	Purified
控制	Includes negative control normal rabbit IgG antibody and control primers specific for the human ARF1 mRNA.
演示	Anti-STAU1 (Staufen 1) (Rabbit Polyclonal),. Lyophilized powder. Add 50 μL of distilled water. Final antibody concentration is 1 mg/ml in PBS buffer. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles. Normal Rabbit IgG. One vial containing 125 µg of purified rabbit IgG in 125 µL of storage buffer containing 0.05% sodium azide. Store at -20°C. RIP Primers, ARF1. One vial containing 75 μL of 5 μM of each primer specific for human ARF1 mRNA. Store at -20°C. FOR: GAC CAC GAT CCT CTA CAA GC REV: TCC CAC ACA GTG AAG CTG ATG

应用
主要应用	Western Blotting
应用说明	Immunoprecipitation from RIP lysate: Representative lot data. RIP lysate from HeLa cells (~2 X 10E7 cell equivalents per IP) were subjected to immunoprecipitation using 5 µg of either a normal rabbit IgG, (Cat. #PP64B), or 5 µg of Anti-STAU1 (Staufen 1) antibody (Cat. # CS204405). Ten percent of the precipitated proteins (lane 1: rabbit IgG, lane 2: STAU1) were resolved by electrophoresis, transferred to nitrocellulose and probed with Anti-STAU1 (Staufen 1) antibody (Cat. # CS204405, 1:1000). Proteins were visualized using One-Step™ IP-Western kit (GenScript Cat. # L00232) . Arrow indicates STAU1. (Figure 2). Western Blot Analysis: Representative lot data. HepG2 cell lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with Anti-STAU1 antibody (Cat. # CS204405, 1:1000 dilution). Proteins were visualized using a Goat Anti-Rabbit IgG conjugated to HRP and a chemiluminescence detection system. Arrow indicates STAU1 (~63 kDa). (Figure 3). Note: Uncharacterized bands appear at ~22 kDa and ~53 kDa in

应用

主要应用

Western Blotting

应用说明

Immunoprecipitation from RIP lysate:
Representative lot data.
RIP lysate from HeLa cells (~2 X 10E7 cell equivalents per IP) were subjected to immunoprecipitation using 5 µg of either a normal rabbit IgG, (Cat. #PP64B), or 5 µg of Anti-STAU1 (Staufen 1) antibody (Cat. # CS204405). Ten percent of the precipitated proteins (lane 1: rabbit IgG, lane 2: STAU1) were resolved by electrophoresis, transferred to nitrocellulose and probed with Anti-STAU1 (Staufen 1) antibody (Cat. # CS204405, 1:1000). Proteins were visualized using One-Step™ IP-Western kit (GenScript Cat. # L00232) .
Arrow indicates STAU1. (Figure 2).
Western Blot Analysis:
Representative lot data.
HepG2 cell lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with Anti-STAU1 antibody (Cat. # CS204405, 1:1000 dilution). Proteins were visualized using a Goat Anti-Rabbit IgG conjugated to HRP and a chemiluminescence detection system.
Arrow indicates STAU1 (~63 kDa). (Figure 3).
Note: Uncharacterized bands appear at ~22 kDa and ~53 kDa in

生物信息
免疫原品种	Synthetic peptide from amino acids 2-51 of human STAU 1.
表位	a.a. 2-51
浓缩	1 mg/mL
宿主	Rat
品种反应性	Human
Species Reactivity Note	Demonstrated to react with human. Other species have not yet been tested.
抗体类型	Polyclonal Antibody
Entrez基因编号	NP_001032405

产品使用声明
质量保证	RNA Binding Protein Immunoprecipitation: RIP Lysate prepared from HeLa cells (2 X 10E7 cell equivalents per IP) were subjected to immunoprecipitation using 5 µg of either a normal Rabbit IgGor 5 µg of Anti-STAU1 antibody and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700). Successful immunoprecipitation of STAU1-associated RNA was verified by qPCR using RIP Primers ARF1, (Figure 1). Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品使用声明

质量保证

RNA Binding Protein Immunoprecipitation:
RIP Lysate prepared from HeLa cells (2 X 10E7 cell equivalents per IP) were subjected to immunoprecipitation using 5 µg of either a normal Rabbit IgGor 5 µg of Anti-STAU1 antibody and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700).
Successful immunoprecipitation of STAU1-associated RNA was verified by qPCR using RIP Primers ARF1, (Figure 1).
Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details.

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

包装信息
数量	10 assays
包装	10 assays per set. Recommended use: ~5 μg of antibody per RIP (dependent upon biological context).

merck millipore,默克密理博,03-116,RIPAb+™ STAU1 (Staufen 1)

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