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merck millipore,默克密理博,07-1799,Anti-NCoA-2 Antibody
产品名称：Anti-NCoA-2 Antibody
产品型号：07-1799
Use Anti-NCoA-2 Antibody (rabbit polyclonal antibody) validated in WB to detect NCoA-2 also known as Transcriptional intermediary factor 2, glucocorticoid receptor-interacting protein-1, nuclear recep More>>
merck millipore,默克密理博,07-1799,Anti-NCoA-2 Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H ChIP, WB Rb Purified Polyclonal Antibody 描述 产品目录编号 07-1799 描述 Anti-NCoA-2 Antibody Alternate Names - transcriptional mediators/intermediary factor 2
- Transcriptional intermediary factor 2
- glucocorticoid receptor-interacting protein-1
- nuclear receptor coactivator 2
- glucocorticoid receptor interacting protein 1
- steroid receptor coactivator-2
背景信息 NCoA2 (nuclear receptor coactivator 2) is a transcriptional coactivator for steroid receptors and nuclear receptors. Also known as glucocorticoid receptor interacting protein 1 (GRIP1), steroid receptor coactivator-2 (SRC-2), or transcriptional mediators/intermediary factor 2 (TIF2), this coregulatory protein contains several nuclear receptor interacting domains and an intrinsic histone acetyltransferase activity. NCoA-2 assists nuclear receptors in the upregulation of DNA expression. In cells ligand activated nuclear receptors recruit NCoA-2 to DNA where it acylates histones to make targeted regions accessible to transcriptional machinery.
产品信息 格式 Purified 控制 - U2 OS cell lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. 应用 应用 Use Anti-NCoA-2 Antibody (rabbit polyclonal antibody) validated in WB to detect NCoA-2 also known as Transcriptional intermediary factor 2, glucocorticoid receptor-interacting protein-1, nuclear receptor coactivator 2. 主要应用 - Chromatin Immunoprecipitation (ChIP)
- Western Blotting
应用说明 Chromatin Immunoprecipitation:
A representative lot of this antibody was used for chIP. See Monroe, D.G., et al. (2006) J. Biol. Chem. 281(39):28596-28604.生物信息 免疫原品种 Linear peptide corresponding to human NCoA-2 表位 Unknown 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes NCoA-2. 同种型 IgG 品种反应性 Human Species Reactivity Note Demonstrated to react with human. Homology to mouse and rat is 95%. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_006531.1
基因符号 - TIF-2
- NCOA2
- GRIP1
- KAT13C
- NCoA-2
- SRC2
- TIF2
- bHLHe75
- hTIF2
- SRC-2
纯化方法 Affinity Purfied UniProt编号 - Q15596
UniProt汇总 FUNCTION: Transcriptional coactivator for steroid receptors and nuclear receptors. Coactivator of the steroid binding domain (AF-2) but not of the modulating N-terminal domain (AF-1). Required with NCOA1 to control energy balance between white and brown adipose tissues.
SUBUNIT STRUCTURE: Present in a complex containing CARM1 and EP300/P300, and interacts with CARM1 and NR3C2 By similarity. Present in a complex containing NCOA3, IKKA, IKKB, IKBKG and CREBBP. Interacts (via C-terminus) with CREBBP. Interacts with HIF1A, NCOA1, APEX and NR3C1. Interacts with CASP8AP2 and TTLL5/STAMP. Interacts with ESR1, RARA and RXRA.
SUBCELLULAR LOCATION: Nucleus.
DOMAIN: Contains four Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. The LXXLL motifs are essential for the association with nuclear receptors and are, at least in part, functionally redundant. Ref.4
The LLXXLXXXL motif is involved in transcriptional coactivation and CREBBP/CBP binding.
Contains 2 C-terminal transcription activation domains (AD1 and AD2) that can function independently.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR By similarity.
INVOLVEMENT IN DISEASE: Chromosomal aberrations involving NCOA2 may be a cause of acute myeloid leukemias. Inversion inv8(p11;q13) generates the MYST3-NCOA2 oncogene, which consists of the N-terminus part of MYST3/MOZ and the C-terminus part of NCOA2/TIF2. MYST3-NCOA2 binds to CREBBP and disrupts its function in transcription activation.
SEQUENCE SIMILARITIES: Belongs to the SRC/p160 nuclear receptor coactivator family.
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAS (PER-ARNT-SIM) domain.产品使用声明 质量保证 Evaluated by Western Blot in U2 OS cell lysate.
Western Blot Analysis: 0.5 µg/ml of this antibody detected SRC2 on 10 µg of U2 OS cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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