merck millipore,默克密理博,07-1720,Anti-FOXO4 Antibody

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merck millipore,默克密理博,07-1720,Anti-FOXO4 Antibody
产品名称：Anti-FOXO4 Antibody
产品型号：07-1720
Anti-FOXO4 Antibody is an antibody against FOXO4 for use in WB & IC.

merck millipore,默克密理博,07-1720,Anti-FOXO4 Antibody

产品介绍

merck millipore,默克密理博,07-1720,Anti-FOXO4 Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	ICC, WB	Rb	Affinity Purified	Polyclonal Antibody

描述
产品目录编号	07-1720
Replaces	MABE539; MABD181
描述	Anti-FOXO4 Antibody
Alternate Names	myeloid/lymphoid or mixed-lineage leukemia (trithorax<br />homolog, Drosophila) Fork head domain transcription factor AFX1 forkhead box O4 myeloid/lymphoid or mixed-lineage leukemia (trithorax<br />(Drosophila) homolog) translocated to, 7 myeloid/lymphoid or mixed-lineage leukemia (trithorax<br />homolog) myeloid/lymphoid or mixed-lineage leukemia (trithorax<br />homolog) myeloid/lymphoid or mixed-lineage leukemia (trithorax<br />homolog
背景信息	Forkhead box O4 (FOXO4 or AFX) is a member of the forkhead family of transcription factors, (FOXO), which play an important role in cellular proliferation, metabolism, stress tolerance, and aging. FOXO transcription factors have a characteristic forkhead domain and are located in the cytoplasm. FOXO4 has similar properties to FOXO1, FOXO3, and FOXO and is associated with acute leukemia. Upon phosphorylation, FOXO4 is translocated from the cytosol to the nucleus. FOXO4 has three phosphorylation sites at Thr32, Ser197, and Ser262. There are 2 pathways, Akt and reversible acetylation, involved in negative control of FOXO4, resulting in cell survival. FOXO4 represses smooth muscle cell (SMC) differentiation by interacting and suppressing myocardin. FOXO4 also down regulates HIF-1 alpha protein levels which prevent hypoxia response.

产品信息
格式	Affinity Purified
控制	Mouse kidney lysate.
演示	Purified rabbit polyclonal antibody precipitated in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.

应用
应用	Anti-FOXO4 Antibody is an antibody against FOXO4 for use in WB & IC.
主要应用	Immunocytochemistry Western Blotting
应用说明	Immunocytochemistry: A 1:500 dilution of this antibody was used to detect FOXO4 in NIH/3T3 and A431 cells. Positive nuclear staining.

生物信息
免疫原品种	Synthetic peptide corresponding to the N-terminal region of FOXO4.
表位	N-terminus
宿主	Rabbit
特异性	This antibody recognizes FOXO4 at the N-terminus.
品种反应性	RatHumanMouse
Species Reactivity Note	Tested on human and mouse. Predicted to react with rat based on sequence homology.
抗体类型	Polyclonal Antibody
Entrez基因编号	NP_005929
基因符号	OTTHUMP00000023496 AFX AFX1 MGC120490 MLLT7
纯化方法	Affinity Purfied
UniProt编号	P98177
UniProt汇总	FUNCTION: Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. Ref.6 Ref.8 Ref.9 Ref.10 Ref.12 SUBUNIT: Interacts with CBP, MYOCD, SIRT1, SRF and YWHAZ. Acetylated by CBP and deacetylated by SIRT1. Binding of YWHAZ inhibits DNA-binding. Ref.10 Ref.12 Ref.11 SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note= When phosphorylated, translocated from nucleus to cytoplasm. Dephosphorylation triggers nuclear translocation. Ref.11 Ref.7 TISSUE SPECIFICITY: Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas. PTM: Acetylation by CBP, which is induced by peroxidase stress, inhibits transcriptional activity. Deacetylation by SIRT1 is NAD-dependent and stimulates transcriptional activity. Phosphorylation by PKB/AKT1 inhibits transcriptional activity and is responsible for cytoplasmic localization. DISEASE: A chromosomal aberration involving FOXO4 is found in acute leukemias. Translocation t(X;11)(q13;q23) with MLL/HRX. The result is a rogue activator protein. Pharmaceutical use A constitutively active FOXO4 mutant where phosphorylation sites Thr-32, Ser-187 and Ser-262 have been mutated to alanine may have therapeutic potential in ERBB2/HER2-overexpressing cancers as it inhibits ERBB2-mediated cell survival, transformation and tumorigenicity. SIMILARITY: Contains 1 fork-head DNA-binding domain.

产品使用声明
质量保证	Western Blot Analysis: 1:500 dilution of this antibody was used to detect FOXO4 in mouse kidney lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at 2-8ºC from date of receipt. To reconstitute the antibody, centrifuge the antibody at a moderate speed (5,000 rpm) for 5 minutes. Carefully remove the ammonium sulfate/PBS buffer solution and discard; 10μL of residual ammonium sulfate solution will not affect the re-suspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur. Re-suspend the antibody pellet in 100L either standard PBS or TBS (pH 7.3-7.5). DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to re-suspend are normal. Vials are overfilled to compensate for any losses.

储存和货运信息

存储条件

Stable for 1 year at 2-8ºC from date of receipt.
To reconstitute the antibody, centrifuge the antibody at a moderate speed (5,000 rpm) for 5 minutes. Carefully remove the ammonium sulfate/PBS buffer solution and discard; 10μL of residual ammonium sulfate solution will not affect the re-suspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur. Re-suspend the antibody pellet in 100L either standard PBS or TBS (pH 7.3-7.5). DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to re-suspend are normal. Vials are overfilled to compensate for any losses.

包装信息
数量	100 µg

merck millipore,默克密理博,07-1720,Anti-FOXO4 Antibody

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