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品种反应性 主要应用 宿主 格式 抗体类型 M, H IHC, WB M Purified Monoclonal Antibody 描述 产品目录编号 05-1946 描述 Anti-USP7 Antibody, clone 1G7 Alternate Names - HAUSP
- ubiquitin specific peptidase 7 (herpes virus-associated)
- Ubiquitin-specific-processing protease 7
- ubiquitin specific protease 7 (herpes virus-associated)
- Herpesvirus-associated ubiquitin-specific protease
- Herpes virus-associated ubiquitin-specific protease
- Ubiquitin thioesterase 7
- ubiquitin specific peptidase 7
- Deubiquitinating enzyme 7
背景信息 Ubiquitin carboxyl-terminal hydrolase 7 (USP7) is a ubiquitin specific protease or a deubiquitylating enzyme that cleaves ubiquitin from its substrates.[Saridakis, 2005] Since ubiquitylation (polyubiquitination) is most commonly associated with the stability and degradation of cellular proteins, Ubiquitin carboxyl-terminal hydrolase 7 activity generally stabilizes its substrate proteins. Ubiquitin carboxyl-terminal hydrolase 7 is most popularly known as a direct antagonist of Mdm2, the E3 ubiquitin ligase for the tumor suppressor protein, p53.[Canning, 2004] 产品信息 格式 Purified 控制 - HeLa cell lysate
演示 Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 This Anti-USP7 Antibody, clone 1G7 is validated for use in WB, IP, IH for the detection of USP7. 主要应用 - Immunohistochemistry
- Western Blotting
生物信息 免疫原品种 Histadine-tagged recombinant protein corresponding to human USP7. 克隆 1G7 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody recognizes USP7. 同种型 IgG1κ 品种反应性 MouseHuman Species Reactivity Note Demonstrated to react with human. Predicted to react with mouse based on 100% sequence homology. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_003461.2
基因符号 - HAUSP
- USP7
- TEF1
纯化方法 Protein G purfied UniProt编号 - Q93009
UniProt汇总 FUNCITON: Cleaves ubiquitin fusion protein substrates. Deubiquitinates TP53/p53 and MDM2 and strongly stabilizes TP53 even in the presence of excess MDM2, and also induces TP53-dependent cell growth repression and apoptosis.
CATALYTIC ACTIVITY: Ubiquitin C-terminal thioester + H2O = ubiquitin + a thiol.
ENZYME REGULATION: Inhibited by N-ethyl-maleimide (NEM) and divalent cations. Tolerates high concentrations of NaCl but is inhibited at concentrations of 195 mM and higher. Ref.7
SUBUNIT STRUCTURE: Monomer. Interacts with TP53, MDM2 and UBXN6. Interacts with herpesvirus 1 trans-acting transcriptional protein ICP0/VMW110 and Epstein-Barr virus EBNA1. EBNA1 shows a 10-fold higher affinity than TP53 and can compete with it for USP7 binding. Binding to VMW110 may modulate the substrate specificity or activity of USP7 to stabilize viral proteins. Interacts with ATXN1 and the strength of interaction is influenced by the length of the poly-Gln region in ATXN1. A weaker interaction seen with mutants having longer poly-Gln regions.
SUBCELLULAR LOCATION: Nucleus. Note: Present in a minority of ND10 nuclear bodies. Association with VMW110 at early times of infection leads to an increased proportion of USP7-containing ND10. Colocalizes with ATXN1 in the nucleus.
TISSUE SPECIFICTY: Widely expressed.
PTM: Polyneddylated (By similarity).
Polyubiquitinated (By similarity).
SEQUENCE SIMILARITIES: Belongs to the peptidase C19 family.
Contains 1 MATH domain.
BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Active from pH 7.0 to 9.5.产品使用声明 质量保证 Evaluated by Western Blot in HeLa lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected USP7 on 10 µg of HeLa cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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