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品种反应性 主要应用 宿主 格式 抗体类型 H WB Rb Purified Polyclonal Antibody 描述 产品目录编号 07-1601 描述 Anti-IRAK1 Antibody, CT Alternate Names - Pelle homolog
- interleukin-1 receptor-associated kinase 1
背景信息 IRAK1, also known as Interleukin-1 receptor associated kinase 1, belongs to the tyrosine kinase related (TKL) family and is involved with mRNA stabilization and activation of NF-kappa B. IRAK1 is located in all tissue as 2 isoforms, with 1 binding quickly and then degrading while isoform 2 is activated by kinase, resulting in a slower more sustained response to cytokines. Phosphorylation of IRAK1 leads to recruitment of the adapter protein PELI1. IRAK1 utilizes 2 pathways, the first one allows for mediation of IL-1 inflammatory responses when TRAF6 is bound, which activates NF-kappa B, thereby inhibiting I kappa B alpha. A second pathway does not involve TRAF6 and results in the stabilization of mRNA. 产品信息 格式 Purified 控制 - Jurkat lysate
演示 Purified rabbit polyclonal in buffer containing PBS and 0.09% sodium azide. 应用 应用 This Anti-IRAK1 Antibody, C-terminus is validated for use in WB for the detection of IRAK1. 主要应用 - Western Blotting
生物信息 免疫原品种 KLH-conjugated synthetic linear peptide corresponding to the C-terminal region of IRAK1. 表位 C-terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes the C-terminal region of IRAK1. 品种反应性 Human Species Reactivity Note Human. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_001020413.1
Entrez基因汇总 This gene encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This gene is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] 基因符号 - EC 2.7.11.1
- IRAK
- IRAK-1
- pelle
纯化方法 Protein G purfied UniProt编号 - P51617
UniProt汇总 Function: Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1.
Catalytic activity: ATP + a protein = ADP + a phosphoprotein.
Cofactor Magnesium.
Subunit structure: IL-1 stimulation leads to the formation of a signaling complex which dissociates from the IL-1 receptor following the binding of PELI1. Interacts with IL1RL1. Interacts with IRAK1BP1 By similarity.
Tissue specificity: Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.
Post-translational modification: Autophosphorylated or is transphosphorylated by IRAK4 following recruitment to the IL-1RI. In the case of isoform 1, this is linked to ubiquitination and degradation.
Sequence similarities: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
Contains 1 protein kinase domain.产品使用声明 质量保证 Evaluated by western blot in jurkat lysate.
Western Blot Analysis: A 1:50-1:500 dilution of this antibody detected IRAK1 on jurkat lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 6 months at 2-8ºC from date of receipt. For long term storage, store at -20ºC.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,23-031M,Cyclin E1 Protein complex, 250 µg
下一件merck millipore产品：merck millipore,默克密理博,116814,Adenosine 3ʹ,5ʹ-cyclic Monophosphorothioate, Rp-Isomer, Triethylammonium Salt - CAS 151837-09-1 - Calbiochem
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