- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
-
-
merck millipore,默克密理博,07-1575,Anti-phospho HDAC1 (Ser421/Ser423) Antibody
产品名称：Anti-phospho HDAC1 (Ser421/Ser423) Antibody
产品型号：07-1575
Use Anti-phospho HDAC1 (Ser421/Ser423) Antibody (Rabbit Polyclonal Antibody) validated in WB to detect phospho HDAC1 (Ser421/Ser423) also known as Histone deacetylase 1, HD1.
merck millipore,默克密理博,07-1575,Anti-phospho HDAC1 (Ser421/Ser423) Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 07-1575 描述 Anti-phospho HDAC1 (Ser421/Ser423) Antibody Alternate Names - HD1
- Histone deacetylase 1
背景信息 Histone deacetylation acts a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylase (HDAC) and histone acetyltransferase (HAT) catalyze this reversible histone acetylation that governs the dynamics of transcriptionally active genes. HDAC1 and HDAC2, belong to class 1 HDAC, contain most of HDAC activity in mammalian cells. HDAC1/2 are core components of multi-protein core repressor complexes containing HDAC1, HDAC2, Rbbp4 and Rbbp7. Phosphorylation of HDAC1/2 enhances enzymatic activity and the association of other proteins. Phosphorylated HDAC1/2 is recruited to a variety of promoters by transcription factors including p53, Rb, YY1, NF-kB, Sp1 and Sp3. Specific phosphorylation of HDAC1 on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes. 产品信息 格式 Affinity Purified 控制 - Nocodazol treated and untreated HeLa acid extract
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Use Anti-phospho HDAC1 (Ser421/Ser423) Antibody (Rabbit Polyclonal Antibody) validated in WB to detect phospho HDAC1 (Ser421/Ser423) also known as Histone deacetylase 1, HD1. 主要应用 - Western Blotting
生物信息 免疫原品种 Linear peptide corresponding to human HDAC1 phosphorylated Ser421/Ser423. 表位 Phosphorylated Ser421/Ser423 宿主 Rabbit 特异性 This antibody recognizes HDAC1 phosphorylated Ser421/Ser423. 品种反应性 Human Species Reactivity Note Demonstrated to react with Human. May recognize Mouse and Rat due to 92% sequence homology with human immunogen. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_004955
Entrez基因汇总 Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq]. 基因符号 - RPD3L1
- HDAC1
修改 - Phosphorylation
纯化方法 Affinity purified UniProt编号 - Q13547
UniProt汇总 FUNCTION: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B.
CATALYTIC ACTIVITY: Hydrolysis of an N(6)-acetyl-lysine residue of a histone to yield a deacetylated histone.
SUBUNIT STRUCTURE: Part of the core histone deacetylase (HDAC) complex composed of HDAC1, HDAC2, RBBP4 and RBBP7. The core complex associates with MTA2, MBD2, MBD3, MTA1L1, CHD3 and CHD4 to form the nucleosome remodeling and histone deacetylation (NuRD) complex, or with SIN3, SAP18 and SAP30 to form the SIN3 HDAC complex. Component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B/BRAF35, KDM1A, RCOR1/CoREST and PHF21A/BHC80. The BHC complex may also contain ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. Associates with the 9-1-1 complex; interacts with HUS1. Found in a complex with DNMT3A and HDAC7. Interacts with BAZ2A/TIP5, BCOR, BRMS1L, DAXX, DNMT1, EP300, HCFC1, NFE4, PCAF, PHB2, MIER1, KDM4A, MINT, NRIP1, PRDM6, RERE, SETDB1, SMYD2, SUV39H1, TGIF, TGIF2, UHRF1, UHRF2 and ZNF541. Interacts with the non-histone region of H2AFY. Interacts with HDAC9. Component of a mSin3A corepressor complex that contains SIN3A, SAP130, SUDS3/SAP45, ARID4B/SAP180, HDAC1 and HDAC2. Interacts with BANP, CBFA2T3 and KDM5B. Interacts with SAP30L. Interacts with E4F1. Interacts with KFL1 By similarity. Interacts with SV40 large T antigen. Interacts with CHFR, PRDM16, SP1, SP3 and SMAD3. Interacts with RB1 and SMARCA4/BRG1. Interacts with TRAF6. Interacts with TSHZ3 (via N-terminus); the interaction is direct. Found in a trimeric complex with APBB1 and TSHZ3; the interaction between HDAC1 and APBB1 is mediated by TSHZ3. Interacts with APEX1; the interaction is not dependent on the acetylated status of APEX1. Interacts with NR4A2/NURR1 and BRMS1 (By similarity).
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICTY: Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.
PTM: Sumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.
Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
Ubiquitinated by CHFR, leading to its degradation by the proteasome (By similarity).
SEQUENCE SIMILARITIES: Belongs to the histone deacetylase family. HD type 1 subfamily.产品使用声明 质量保证 Evaluated by Western Blot in Nocodazol treated and untreated HeLa acid extract.
Western Blot Analysis: 1:1,000 dilution of this antibody detected HDAC1 on 10 µg of Nocodazol treated and untreated HeLa acid extract.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µL
上一件merck millipore产品：merck millipore,默克密理博,05-690,Anti-HP1γ Antibody, clone 42s2
下一件merck millipore产品：merck millipore,默克密理博,07-013,Anti-MeCP2 Antibody
相关产品
merck millipore,默克密理博,219498,Cathepsin S Substrate, Fluorogenic - Calbiochem
merck millipore,默克密理博,14-1061,Human Recombinant Hp1γ (CBX3)
merck millipore,默克密理博,205546,Caffeic Acid - CAS 331-39-5 - Calbiochem
merck millipore,默克密理博,MABN643,Anti-NDUFS3 Antibody, clone 6H7.1
merck millipore,默克密理博,05-690,Anti-HP1γ Antibody, clone 42s2
merck millipore,默克密理博,07-013,Anti-MeCP2 Antibody
merck millipore,默克密理博,SF-3098,INSR; Human, Cyanine 5 RNA Detection Probe,SmartFlare
merck millipore,默克密理博,104850,Actin Polymerization Interfering Agents Set - Calbiochem
merck millipore,默克密理博,AB9774-50UL,Anti-Somatostatin Receptor Type 3 Antibody