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merck millipore,默克密理博,05-690,Anti-HP1γ Antibody, clone 42s2
产品名称：Anti-HP1γ Antibody, clone 42s2
产品型号：05-690
Use Anti-HP1γ Antibody, clone 42s2 (Mouse Monoclonal Antibody) validated in ChIP, IHC, WB to detect HP1γ also known as Heterochromatin protein 1 homolog gamma.
merck millipore,默克密理博,05-690,Anti-HP1γ Antibody, clone 42s2
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M, H ChIP, IHC, WB M Purified Monoclonal Antibody 描述 产品目录编号 05-690 品牌系列 Upstate
商名 - Upstate
描述 Anti-HP1γ Antibody, clone 42s2 Alternate Names - heterochromatin-like protein 1
- HP1 gamma
- HP1 gamma homolog
- Heterochromatin protein 1 homolog gamma
- Modifier 2 protein
- chromobox homolog 3
- chromobox homolog 3 (Drosophila HP1 gamma)
- chromobox homolog 3 (HP1 gamma homolog, Drosophila)
- heterochromatin protein HP1 gamma
- heterochromatin protein HP1 gamma
背景信息 Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication. The p150 subunit of CAF-1 also supports the maintenance of heterochromatin, which requires the synthesis of both new histones and heterochromatin proteins and their orderly assembly during DNA replication.
Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated产品信息 格式 Purified 控制 - HeLa acid extracts, HEK 293 nuclear extracts.
演示 Purified mouse monoclonal IgG1 in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175M NaCl, 0.07% sodium azide, and 30% glycerol. Liquid at -20ºC. 应用 应用 Use Anti-HP1γ Antibody, clone 42s2 (Mouse Monoclonal Antibody) validated in ChIP, IHC, WB to detect HP1γ also known as Heterochromatin protein 1 homolog gamma. 主要应用 - Chromatin Immunoprecipitation (ChIP)
- Immunohistochemistry
- Western Blotting
应用说明 Immunohistochemistry:
This antibody has been reported by an independent laboratory to detect HP1γ positive cells in breast tissue.
Immunoprecipitation/Chromatin Immunoprecipitation:
This antibody has been reported by an independent laboratory to immunoprecipitate HP1γ from nuclear extracts and formalin-cross-linked chromatin.生物信息 免疫原品种 GST fusion protein corresponding to residues 17-173 of human HP1γ protein. Clone 42s2. 克隆 42s2 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody recognizes HP1γ protein, Mr ~25 kDa. 同种型 IgG1 品种反应性 MouseHuman Species Reactivity Note Reacts with human, mouse. Reactivity with other species has not been determined. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_009207
Entrez基因汇总 At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene.
Transcript Variant: This variant (1) contains an alternate 5' exon but encodes the same protein as transcript variant 2.基因符号 - HP1Hs-gamma
- CBX3
- HECH
- HP1-GAMMA
纯化方法 Protein G purfied UniProt编号 - Q13185
UniProt汇总 FUNCTION: SwissProt: Q13185 # Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
SIZE: 183 amino acids; 20811 Da
SUBUNIT: Binds directly to CHAF1A. Interacts with histone H3 methylated at 'Lys-9'. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2, MBLR, L3MBTL2 and YAF2. Interacts with LBR, INCENP, TRIM28/TIF1B, SUV420H1, SUV420H2 and SP100. Interacts with TIF1A (By similarity). Interacts with MIS12 and C20orf127. Can interact directly with CBX5 via the chromoshadow domain.
SUBCELLULAR LOCATION: Nucleus (Potential). Note=Associates with euchromatin and is largely excluded from constitutive heterochromatin. May be associated with microtubules and mitotic poles during mitosis (Potential).
PTM: Phosphorylated by PIM1. Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
SIMILARITY: SwissProt: Q13185 ## Contains 2 chromo domains.产品使用声明 质量保证 Routinely evaluated by western blot in acid-extracted HeLa cell lysates (Catalog #13-112).
Western Blot Analysis:
0.01-0.1 µg/mL of this lot detected HP1γ protein in acid-extracted HeLa cell lysates (Catalog # 13-112).销售限制 Due to license agreement restrictions, this product cannot be purchased for resale. 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. 包装信息 数量 200 µg
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