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Replacement Information Replacement Information 07-1402 is a recommended replacement for AB1342 重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, M ELISA, WB Rb Serum Polyclonal Antibody 描述 产品目录编号 07-1402 Replaces AB1342 描述 Anti-GLUT-2 Antibody Alternate Names - Solute carrier family 2, facilitated glucose transporter, member 2
- Glucose Transporter type 2, liver
- liver
- Solute carrier family 2
- facilitated glucose transporter
背景信息 GLUTs (Glucose transporters) are a family of integral membrane proteins that facilitative glucose uptake by cells. They are integral membrane glycoproteins involved in transporting glucose into most cells. Seven types of glucose transport carrier proteins, designated as GLUT 1 to 7, facilitate glucose transport across the cell membrane. Molecular cloning of glucose transporters have identified a family of closely related genes that encode at least 7 proteins exhibiting high degree of amino acid homology (45% to 65%), all in the molecular weight range of 40 to 60 kDa. GLUT-1 functions in constitutive or basal glucose uptake in adult tissues and is the predominant glucose transporter in embryonic and fetal tissues. It has two forms, a 55kDa and a 45 kDa. The 55kDa form of GLUT1 regulates import of glucose from blood to brain across the endothelial cells of the blood-brain barrier (BBB), whereas the 45 kDa form of GLUT1 predominantly regulates nonvascular glial glucose uptake. GLUT2 is a facilitative glucose transporter. This isoform likely mediates the bidirectional transfer of glucose across the plasma membrane of hepatocytes and is responsible for uptake of glucose by the beta cells; may comprise part of the glucose-sensing mechanism of the beta cell. May also participate with the Na(+)/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney. GLUT-4 is present in skeletal muscle, heart and adipose tissues. GLUT-4 is highly conserved between species. The expression of GLUT-4 is greatly influenced by insulin and other stimulators of the IP3 signaling pathway. Binding of insulin to the insulin receptor signals through PI3K and Akt to translocate GLUT-4 to the plasma membrane where it allows the uptake of glucose. 产品信息 格式 Serum 控制 - HEK293 cell lysate
演示 Rabbit polyclonal antiserum in buffer containing 0.05% sodium azide. 应用 应用 This Anti-GLUT-2 Antibody is validated for use in WB, ELISA for the detection of GLUT-2. 主要应用 - ELISA
- Western Blotting
应用说明
ELISA: A previous lot of this antibody was used on ELISA at 1:10,000 - 1:50,000 dilution.
Western Blot: A previous lot of this antibody was tested on multiple lysates. See datasheet生物信息 免疫原品种 A 16 amino acid peptide sequence corresponding to the exoplasmic loop between helices 1 and 2 of rat liver Glucose Transporter coupled to KLH. 宿主 Rabbit 特异性 Recognizes GLUT-2 in mouse, rat, and human tissues. 同种型 IgG 品种反应性 RatHumanMouse Species Reactivity Note Human, Mouse, Rat 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_000340.1
Entrez基因汇总 Glucose transporter 2 isoform is an integral plasma membrane glycoprotein of the liver, islet beta cells, intestine, and kidney epithelium. It mediates facilitated bidirectional glucose transport. Because of its low affinity for glucose, it has been suggested as a glucose sensor. [provided by RefSeq]
基因符号 - GLUT2
- SLC2A2
- GLUT-2
纯化方法 Unpurified UniProt编号 - P11168
UniProt汇总 FUNCTION: Facilitative glucose transporter. This isoform likely mediates the bidirectional transfer of glucose across the plasma membrane of hepatocytes and is responsible for uptake of glucose by the beta cells; may comprise part of the glucose-sensing mechanism of the beta cell. May also participate with the Na(+)/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Liver, insulin-producing beta cell, small intestine and kidney.
PTM: N-glycosylated; required for stability and retention at the cell surface of pancreatic beta cells (By similarity).
DISEASE: Defects in SLC2A2 are the cause of Fanconi-Bickel syndrome (FBS) [MIM:227810]. FBS is a rare, well-defined clinical entity, inherited in an autosomal recessive mode and characterized by hepatorenal glycogen accumulation, proximal renal tubular dysfunction, and impaired utilization of glucose and galactose.
SIMILARITY: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily [view classification].
产品使用声明 质量保证 Routinely evaluated by Western Blot on HEK293 lysates.
Western Blot Analysis:
1:500-1:1,500 dilution of this lot detected GLUT-2 on 10 µg of HEK293 lysates.
使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µL
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